Mouse Cytokine Array (62 targets) (SARB0081)

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SKU:
SARB0081
Product Type:
Protein Array
Analytes:
Axl
Analytes:
BLC (CXCL13)
Analytes:
CD30 Ligand (TNFSF8)
Analytes:
CD30 (TNFRSF8)
Analytes:
CD40 (TNFRSF5)
Analytes:
CRG-2
Analytes:
CTACK (CCL27)
Analytes:
CXCL16
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Fas Ligand (TNFSF6)
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCSF
Analytes:
GM-CSF
Analytes:
IFN-gamma
Analytes:
IGFBP-3
Analytes:
IGFBP-5
Analytes:
IGFBP-6
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-10
Analytes:
IL-12 p40/p70
Analytes:
IL-12 p70
Analytes:
IL-13
Analytes:
IL-17A
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-2
Analytes:
IL-3
Analytes:
IL-3 R beta
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-6
Analytes:
IL-9
Analytes:
KC (CXCL1)
Analytes:
Leptin
Analytes:
Leptin R
Analytes:
LIX
Analytes:
L-Selectin (CD62L)
Analytes:
Lymphotactin (XCL1)
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-5
Analytes:
M-CSF
Analytes:
MIG (CXCL9)
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MIP-1 gamma
Reactivity:
Mouse
Applications:
Multiplex Array
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Description

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Mouse Cytokine Array (62 targets) (SARB0081)

The Mouse Cytokine Array (SARB0081) is a powerful tool for researchers studying cytokine expression in mouse samples. This array kit allows for the simultaneous detection of 62 different cytokines, providing a comprehensive view of the immune response in a single experiment.With its high sensitivity and specificity, this cytokine array enables accurate quantification of cytokine levels in various biological samples, including serum, plasma, cell culture supernatants, and tissue lysates. The array is easy to use, making it suitable for both experienced researchers and those new to cytokine analysis.

By utilizing the Mouse Cytokine Array, researchers can gain valuable insights into the molecular mechanisms underlying immune responses, inflammation, and disease pathology. This array kit is an essential tool for studies in immunology, cancer research, and other fields where cytokine profiling is essential for understanding disease processes and developing new therapeutic strategies.

Product Sku:SARB0081
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:62
Gene Symbols:AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL24, CCL25|CCL27, CCL3, CCL5, CCL9, CD40, CSF1, CSF2, CSF2RB, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL9, FASLG, IFNG, IGFBP3, IGFBP5, IGFBP6, IL10, IL12A, IL13, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, LEPR, PF4, SELL, SELP, THPO, TIMP1, TNF, TNFRSF8, TNFSF8, TPO, VCAM1, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Axl
  • Gene Symbol: Axl
  • Gene ID: N/A
  • Accession #: Q00993
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: N/A
  • Accession #: O55038
CD30 Ligand (TNFSF8)
  • Gene Symbol: CD30LG
  • Gene ID: N/A
  • Accession #: P32972
CD30 (TNFRSF8)
  • Gene Symbol: CD30
  • Gene ID: N/A
  • Accession #: Q60846
CD40 (TNFRSF5)
  • Gene Symbol: CD40
  • Gene ID: N/A
  • Accession #: Q99NE2
CRG-2
  • Gene Symbol: Cxcl10
  • Gene ID: N/A
  • Accession #: P17515
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Gene ID: N/A
  • Accession #: Q9Z1X0
CXCL16
  • Gene Symbol: CXCL16
  • Gene ID: N/A
  • Accession #: Q5F2D5
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: N/A
  • Accession #: P48298
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: N/A
  • Accession #: Q9JKC0
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG
  • Gene ID: N/A
  • Accession #: P41047
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: N/A
  • Accession #: O35188
GCSF
  • Gene Symbol: CSF3
  • Gene ID: N/A
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: N/A
  • Accession #: P26955
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: N/A
  • Accession #: P01580
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: N/A
  • Accession #: P47878
IGFBP-5
  • Gene Symbol: IGFBP5
  • Gene ID: N/A
  • Accession #: Q07079
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: N/A
  • Accession #: Q91X24
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: N/A
  • Accession #: P10749
IL-10
  • Gene Symbol: IL10
  • Gene ID: N/A
  • Accession #: Q0VBJ1
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Gene ID: N/A
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: N/A
  • Accession #: P43431
IL-13
  • Gene Symbol: IL13
  • Gene ID: N/A
  • Accession #: P20109
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: N/A
  • Accession #: Q60971
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: N/A
  • Accession #: P01582
IL-2
  • Gene Symbol: IL2
  • Gene ID: N/A
  • Accession #: Q791T3
IL-3
  • Gene Symbol: IL3
  • Gene ID: N/A
  • Accession #: P01586
IL-3 R beta
  • Gene Symbol: Csf2rb
  • Gene ID: N/A
  • Accession #: P26955
IL-4
  • Gene Symbol: IL4
  • Gene ID: N/A
  • Accession #: P07750
IL-5
  • Gene Symbol: IL5
  • Gene ID: N/A
  • Accession #: P04401
IL-6
  • Gene Symbol: IL6
  • Gene ID: N/A
  • Accession #: P08505
IL-9
  • Gene Symbol: IL9
  • Gene ID: N/A
  • Accession #: P15247
KC (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: N/A
  • Accession #: P12850
Leptin
  • Gene Symbol: LEP
  • Gene ID: N/A
  • Accession #: P41160
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: N/A
  • Accession #: Q61215
LIX
  • Gene Symbol: Cxcl5
  • Gene ID: N/A
  • Accession #: P50228
L-Selectin (CD62L)
  • Gene Symbol: SELL
  • Gene ID: N/A
  • Accession #: Q3TCF3
Lymphotactin (XCL1)
  • Gene Symbol: XCL1
  • Gene ID: N/A
  • Accession #: P47993
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: N/A
  • Accession #: Q9QYD7
MCP-5
  • Gene Symbol: Ccl12
  • Gene ID: N/A
  • Accession #: Q9QYD6
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: N/A
  • Accession #: P07141
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: N/A
  • Accession #: P18340
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: N/A
  • Accession #: P14096
MIP-1 gamma
  • Gene Symbol: Ccl9
  • Gene ID: N/A
  • Accession #: Q5QNW2
MIP-2
  • Gene Symbol: Cxcl2
  • Gene ID: N/A
  • Accession #: P10889
MIP-3 beta (CCL19)
  • Gene Symbol: CCL19
  • Gene ID: N/A
  • Accession #: O70460
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: N/A
  • Accession #: O89093
Platelet Factor 4 (CXCL4)
  • Gene Symbol: PF4, CXCL4, SCYB4
  • Gene ID: N/A
  • Accession #: Q9Z126
P-Selectin
  • Gene Symbol: SELP
  • Gene ID: N/A
  • Accession #: Q01102
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: N/A
  • Accession #: P30882
SCF
  • Gene Symbol: KITLG
  • Gene ID: N/A
  • Accession #: P20826
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: N/A
  • Accession #: P40224
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: N/A
  • Accession #: P25118
TNF RII (TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: N/A
  • Accession #: O88734
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: N/A
  • Accession #: Q9WUZ6
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: N/A
  • Accession #: P14098
TECK (CCL25)
  • Gene Symbol: CCL25
  • Gene ID: N/A
  • Accession #: O35903
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: N/A
  • Accession #: P12032
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: N/A
  • Accession #: Q62326
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: N/A
  • Accession #: P40226
VCAM-1 (CD106)
  • Gene Symbol: VCAM1
  • Gene ID: N/A
  • Accession #: P29533
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: N/A
  • Accession #: Q00731
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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