Description
Mouse Cytokine Array (32 targets) (SARB0080)
The Mouse Cytokine Array (32 targets) (SARB0080) is a powerful tool designed for researchers studying cytokine networks and immune responses in mouse models. This array allows for the simultaneous detection of 32 different cytokines and chemokines in a single sample, providing comprehensive data on the immune profile of cells or tissues.With high sensitivity and specificity, this array enables researchers to accurately measure the levels of various cytokines, including TNF-alpha, IL-6, and IFN-gamma, among others. The array is easy to use and provides reliable results, making it ideal for a wide range of applications, from basic research to drug discovery and development.
By using the Mouse Cytokine Array (32 targets) (SARB0080), researchers can gain valuable insights into the complex interplay of cytokines and chemokines in immune responses, inflammation, and disease pathogenesis. This versatile tool can help accelerate discoveries in immunology, oncology, and inflammatory diseases, providing vital information for the development of new therapeutic strategies.
| Product Sku: | SARB0080 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 32 |
| Gene Symbols: | CCL11, CCL12, CCL17, CCL19, CCL2, CCL20, CCL21, CCL27, CCL3, CCL5|CSF2, CSF3, CXCL1, CXCL2, IFNG, IL10, IL12A, IL13, IL2, IL3, IL4, IL5, IL6, IL9, KITLG, LEP, THPO, TIMP1, TNF, TNFRSF1A, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
6Ckine (CCL21)
| CTACK (CCL27)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IL-2
| IL-3
| IL-4
| IL-5
| IL-6
|
IL-9
| IL-10
| IL-12 p40/p70
| IL-12 p70
| IL-13
|
IL-17A
| IFN-gamma
| KC (CXCL1)
| Leptin
| MCP-1 (CCL2)
|
MCP-5
| MIP-1 alpha (CCL3)
| MIP-2
| MIP-3 beta (CCL19)
| RANTES (CCL5)
|
SCF
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TIMP-1
| TNF alpha
|
Thrombopoietin (TPO)
| VEGF-A
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
