Description
system_update_altDatasheet
Mouse CD6 PharmaGenie ELISA Kit
| Product SKU: | SBRS1310 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BAFF R, Betacellulin (BTC), C5a, CCL6, CD48 (SLAMF2), CD80 (B7-1), Chemerin, Clusterin, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33 (IL-1 F11), IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2 (NGAL), LOX-1, Lungkine (CXCL15), Marapsin, MBL-2, Meteorin, Nope, NOV (CCN3), Osteoactivin (GPNMB), OX40 Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1 (KIM-1), TRAIL (TNFSF10), Tryptase epsilon. |
| Uniprot: | Q61003 |
| Gene ID: | 12511 |
| Gene Names: | CD6 |
| Synonyms: | T-cell differentiation antigen CD6 (CD antigen CD6) |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.7 pg/ml -150 pg/ml |
| Sensitivity: | 0.7 pg/ml |
| Recommended Dilution: | 10 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Mouse CD6 PharmaGenie ELISA Kit (SBRS1310) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
