Description
| Product Name: | Mouse BCA-1 Recombinant Protein |
| Product Code: | RPPB1100 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | BCA-1 |
| Synonyms: | C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, Cxcl13, Blc, Scyb13. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The BCA1 protein was lyophilized from a 0.2�m filtered solution in Acetonitrile and TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized CXCL13 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA |
| Biological Activity: | The ED50, as measured by its ability to chemoattract human CXCR5-transfected mouse BaF3 cells, is less than 2�g/ml. |
BCA-1 is a CXC chemokine that is highly expressed in thesecondary lymphoid organs, such as follicles of the spleen, lymph nodes, and Peyer's patches. CXCL13 promotes the migration of B lymphocytes (compared to T cells and macrophages), by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). BCA1 therefore function in the homing of B lymphocytes to follicles. Human BCA-1 shares a 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt�s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR-1, now designated CXCR-5, is restricted to B lymphocytes and a subpopulation of T helper memory cells.
CXCL13 Mouse Recombinant (22-109) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10kDa.The BCA-1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CXCL13: Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5. Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Protein Details: | Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide Cellular Component: extracellular space; cell; extracellular region; cytosol Molecular Function:heparin binding; fibroblast growth factor binding; CXCR3 chemokine receptor binding; chemokine activity; protein heterodimerization activity; CXCR5 chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity; receptor agonist activity Biological Process: lymphocyte chemotaxis across high endothelial venule; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; chemotaxis; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of integrin activation; lymph node development; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; defense response to bacterium; immune response; inflammatory response |
| UniProt Code: | O55038 |
| NCBI GenInfo Identifier: | 7673946 |
| NCBI Gene ID: | 55985 |
| NCBI Accession: | O55038.1 |
| UniProt Related Accession: | O55038 |
| Molecular Weight: | |
| NCBI Full Name: | C-X-C motif chemokine 13 |
| NCBI Synonym Full Names: | chemokine (C-X-C motif) ligand 13 |
| NCBI Official Symbol: | Cxcl13�� |
| NCBI Official Synonym Symbols: | BLC; Angie; BCA-1; BLR1L; ANGIE2; Scyb13; 4631412M08Rik�� |
| NCBI Protein Information: | C-X-C motif chemokine 13; CXC chemokine BLC; B lymphocyte chemoattractant; small-inducible cytokine B13; small inducible cytokine subfamily B (Cys-X-Cys), member 13 |
| UniProt Protein Name: | C-X-C motif chemokine 13 |
| UniProt Synonym Protein Names: | B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13 |
| Protein Family: | Beta carbonic anhydrase |
| UniProt Gene Name: | Cxcl13�� |
| UniProt Entry Name: | CXL13_MOUSE |
