Description
system_update_altDatasheet
Mouse Adiponectin PharmaGenie ELISA Kit
| Product SKU: | SBRS1273 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS-1, Angiopoietin-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGFbeta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2. |
| Uniprot: | Q60994 |
| Gene ID: | 11450 |
| Gene Names: | ACDC / ACRP30 / ADIPOQ / APM1 |
| Synonyms: | Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipocyte-specific protein AdipoQ) |
| Target Species: | Mouse |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 49 pg/ml - 12,000 pg/ml |
| Sensitivity: | 49 pg/ml |
| Recommended Dilution: | 20,000 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Mouse Adiponectin PharmaGenie ELISA Kit (SBRS1273) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
