Description
| Product Name: | MERS-CoV NucleoRecombinant Protein / NP Recombinant Protein (His Tag) |
| Product Code: | RPES6985 |
| Size: | 100µg |
| Species: | MERS-CoV |
| Expression Host: | Baculovirus-Insect Cells |
| Synonyms: | coronavirus NP Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, cov np Protein, MERS-CoV, ncov NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, NP Protein, MERS-CoV, Nucleocapsid Protein, MERS-CoV, Nucleoprotein Protein, MERS-CoV |
| Mol Mass: | 46.51kDa. |
| Tag: | C-His |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin Level: | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Bio Activity: | Testing in progress |
| Sequence: | Met1-Asp413 |
| Accession: | AFS88943.1 |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
| Background: | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
