Description
| 抗体名: | MAPKAP1 Antibody (PACO43291) |
| 抗体コード: | PACO43291 |
| サイズ: | 50ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:2000 |
| 反応性: | Human |
| 免疫原: | Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1 protein (1-290AA) |
| 憲法: | Liquid |
| ストレージバッファ: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 精製方法: | Antigen Affinity Purified |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: Target of rapamycin complex 2 subunit MAPKAP1 antibody at 2µg/ml. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60, 55, 54, 38, 37, 42 kDa. Observed band size: 60 kDa. |
| バックグラウンド: | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. |
| シノニム: | Target of rapamycin complex 2 subunit MAPKAP1 (TORC2 subunit MAPKAP1) (Mitogen-activated protein kinase 2-associated protein 1) (Stress-activated map kinase-interacting protein 1) (SAPK-interacting protein 1) (mSIN1), MAPKAP1, MIP1 SIN1 |
| UniProt Protein Function: | Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 9q33.3 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding Biological Process: epidermal growth factor receptor signaling pathway; substantia nigra development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; T cell costimulation; innate immune response; negative regulation of Ras protein signal transduction; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BPZ7 |
| NCBI GenInfo Identifier: | 15214282 |
| NCBI Gene ID: | 79109 |
| NCBI Accession: | Q9BPZ7.2 |
| UniProt Related Accession: | Q9BPZ7 |
| Molecular Weight: | Observed: 59 kDaPredicted: 60 kDa |
| NCBI Full Name: | Target of rapamycin complex 2 subunit MAPKAP1 |
| NCBI Synonym Full Names: | MAPK associated protein 1 |
| NCBI Official Symbol: | MAPKAP1 |
| NCBI Official Synonym Symbols: | MIP1; SIN1; JC310; SIN1b; SIN1g |
| NCBI Protein Information: | target of rapamycin complex 2 subunit MAPKAP1 |
| UniProt Protein Name: | Target of rapamycin complex 2 subunit MAPKAP1 |
| UniProt Synonym Protein Names: | Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1 |
| Protein Family: | Stress-activated map kinase-interacting protein |
| UniProt Gene Name: | MAPKAP1 |
| UniProt Entry Name: | SIN1_HUMAN |
