Description
| 抗体名: | LIMK2 Antibody (PACO06614) |
| 抗体コード: | PACO06614 |
| サイズ: | 50ug |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | ELISA:1:40000, WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000 |
| 反応性: | Human, Mouse, Rat |
| 免疫原: | Synthesized peptide derived from the Internal region of human LIMK-2. |
| 憲法: | Liquid |
| ストレージバッファ: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| 精製方法: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot analysis of Jurkat cells using LIMK-2 Polyclonal Antibody. |
 | Western Blot analysis of HuvEc cells using LIMK-2 Polyclonal Antibody. |
| シノニム: | LIMK2; LIM domain kinase 2; LIMK-2 |
| UniProt Protein Function: | LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified. |
| UniProt Protein Details: | Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cis-Golgi network; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation |
| NCBI Summary: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P53671 |
| NCBI GenInfo Identifier: | 1708824 |
| NCBI Gene ID: | 3985 |
| NCBI Accession: | P53671.1 |
| UniProt Secondary Accession: | P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5 |
| UniProt Related Accession: | P53671 |
| Molecular Weight: | 638 |
| NCBI Full Name: | LIM domain kinase 2 |
| NCBI Synonym Full Names: | LIM domain kinase 2 |
| NCBI Official Symbol: | LIMK2 |
| NCBI Protein Information: | LIM domain kinase 2 |
| UniProt Protein Name: | LIM domain kinase 2 |
| Protein Family: | LIM domain kinase |
| UniProt Gene Name: | LIMK2 |
| UniProt Entry Name: | LIMK2_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
