Description
| 抗体名: | LIG4 Antibody (PACO22061) |
| 抗体コード: | PACO22061 |
| サイズ: | 100ul |
| 宿主種: | Rabbit |
| 申し込み: | ELISA, WB |
| 推奨される希釈: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| 反応性: | Human |
| 免疫原: | Synthesized peptide derived from internal of human DNL4. |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 抗体のクローン性: | Polyclonal |
| アイソタイプ: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from Jurkat cells, using DNL4 antibody. |
| バックグラウンド: | Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. |
| シノニム: | DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4 |
| UniProt Protein Function: | LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome. |
| UniProt Protein Details: | Protein type:EC 6.5.1.1; Ligase Chromosomal Location of Human Ortholog: 13q33-q34 Cellular Component: condensed chromosome; cytoplasm; DNA ligase IV complex; DNA-dependent protein kinase complex; focal adhesion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane Molecular Function:ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; ligase activity; metal ion binding; protein binding; protein C-terminus binding Biological Process: cell cycle; cell division; cell proliferation; central nervous system development; chromosome organization and biogenesis; DNA ligation; DNA ligation during DNA recombination; DNA ligation during DNA repair; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; lagging strand elongation; negative regulation of neuron apoptosis; neuron apoptosis; nucleotide-excision repair, DNA gap filling; positive regulation of fibroblast proliferation; positive regulation of neurogenesis; pro-B cell differentiation; response to gamma radiation; response to X-ray; single strand break repair; somatic stem cell maintenance; T cell differentiation in the thymus; T cell receptor V(D)J recombination; V(D)J recombination; viral reproduction Disease: Lig4 Syndrome; Myeloma, Multiple |
| NCBI Summary: | The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49917 |
| NCBI GenInfo Identifier: | 88911290 |
| NCBI Gene ID: | 3981 |
| NCBI Accession: | P49917.2 |
| UniProt Secondary Accession: | P49917,Q8IY66, Q8TEU5, |
| UniProt Related Accession: | P49917 |
| Molecular Weight: | 103,971 Da |
| NCBI Full Name: | DNA ligase 4 |
| NCBI Synonym Full Names: | ligase IV, DNA, ATP-dependent |
| NCBI Official Symbol: | LIG4 |
| NCBI Official Synonym Symbols: | LIG4S |
| NCBI Protein Information: | DNA ligase 4 |
| UniProt Protein Name: | DNA ligase 4 |
| UniProt Synonym Protein Names: | DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4 |
| Protein Family: | DNA ligase |
| UniProt Gene Name: | LIG4 |
| UniProt Entry Name: | DNLI4_HUMAN |
| Antibodies |
| Anti-LIG4 Antibody (CAB1743) |
| Anti-DNA Ligase IV Antibody (CAB11432) |
| Anti-LIG4 Antibody (CAB18050)[KO Validated] |
| Phospho-LIG4 (T650) Antibody (PACO04236) |
| LIG4 Antibody (PACO04237) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
