The IBA57 Polyclonal Antibody (PACO60092) is a vital tool for researchers studying IBA57, a key protein involved in mitochondrial function and iron-sulfur cluster biogenesis. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blotting and immunofluorescence applications. By targeting the IBA57 protein, researchers can analyze its expression and localization in different cell types, enabling a deeper understanding of its role in mitochondrial health and disease.
IBA57 is essential for the assembly of iron-sulfur clusters, which are critical cofactors for various enzymes involved in mitochondrial metabolism and electron transport chain function. Dysregulation of IBA57 has been linked to mitochondrial disorders and neurodegenerative diseases, making it a promising target for therapeutic interventions. By studying the functions of IBA57 using this antibody, researchers can uncover new insights into mitochondrial biology and potentially develop novel treatments for associated disorders.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, Mouse liver tissue. All lanes: IBA57 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
IHC image of PACO60092 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO60092 diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.
The protein encoded by this gene localizes to the mitochondrion and is part of the iron-sulfur cluster assembly pathway. The encoded protein functions late in the biosynthesis of mitochondrial 4Fe-4S proteins. Defects in this gene have been associated with autosomal recessive spastic paraplegia-74 and with multiple mitochondrial dysfunctions syndrome-3. Two transcript variants encoding different isoforms have been found for this gene. The smaller isoform is not likely to be localized to the mitochondrion since it lacks the amino-terminal transit peptide. [provided by RefSeq, Jul 2015]
