Description
| Product Name: | Human ZWINT Recombinant Protein |
| Product Code: | RPPB5191 |
| Size: | 10µg |
| Species: | Human |
| Target: | ZWINT |
| Synonyms: | ZW10 Interacting Kinetochore Protein, ZWINT, ZW10 Interactor, HZwint-1, KNTC2AP, ZWINT1, Human ZW10 Interacting Protein-1, ZW10 Interactor Kinetochore Protein zwint-1, Zwint-1, ZW10-Interacting Protein 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ZWINT solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP |
ZW10 Interacting Kinetochore Protein (ZWINT) is involved in kinetochore function. ZWINT is part of the MIS12 complex, which is essential for kinetochore formation and spindle checkpoint activity. ZWINT is localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase. ZWINT interacts with ZW10 (Zeste White 10) and regulates the association between ZW10 and kinetochores. ZWINT gene defects are linked with the pathogenesis of Roberts�s syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation.
ZWINT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27.9 kDa (Molecular size on SDS-PAGE will appear higher).ZWINT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q21-q22 Cellular Component: cytoplasm; cytosol; kinetochore Molecular Function:protein binding; protein N-terminus binding Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95229 |
| NCBI GenInfo Identifier: | 117949386 |
| NCBI Gene ID: | 11130 |
| NCBI Accession: | O95229.2 |
| UniProt Secondary Accession: | O95229,Q0D2I3, Q9BWD0, A6NNV6, |
| UniProt Related Accession: | O95229 |
| Molecular Weight: | 25,544 Da |
| NCBI Full Name: | ZW10 interactor |
| NCBI Synonym Full Names: | ZW10 interacting kinetochore protein |
| NCBI Official Symbol: | ZWINT�� |
| NCBI Official Synonym Symbols: | SIP30; ZWINT1; KNTC2AP; HZwint-1�� |
| NCBI Protein Information: | ZW10 interactor |
| UniProt Protein Name: | ZW10 interactor |
| UniProt Synonym Protein Names: | ZW10-interacting protein 1; Zwint-1 |
| Protein Family: | ZW10 interactor |
| UniProt Gene Name: | ZWINT�� |
| UniProt Entry Name: | ZWINT_HUMAN |
