Description
| Product Name: | Human VEGF D Recombinant Protein |
| Product Code: | RPPB1087 |
| Size: | 10µg |
| Species: | Human |
| Target: | VEGF D |
| Synonyms: | c-fos induced growth factor (vascular endothelial growth factor D), FIGF, VEGFD. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The recombinant VEGF-D was lyophilized after extensive dialysis against PBS. |
| Solubility: | It is recommended to reconstitute the Vascular Endothelial Growth Factor D in sterile 18M-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized VEGF-D although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-D should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity: | The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of human microvascular endothelial cells (HMVECs). |
VEGF-D belongs to the VEGF/PDGF family of proteins. VEGF-D promotes lymphangiogesis, endothelial cell growth, and regulates vascular permeability. In addition, VEGF-D has an important part in the creation of the venous and lymphatic vascular systems and in the growth and maintenance of differentiated lymphatic endothelium Mature VEGF-D forms a noncovalently linked homodimer, and binds to and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4).
VEGFD Human Recombinant produced in HEK-293 cells is a secreted protein (amino acids Phe93-Ser201) fused to a polyhistidine tag at the C-terminus.
| UniProt Protein Function: | VEGFD: Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Belongs to the PDGF/VEGF growth factor family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted; Cytokine; Secreted, signal peptide Chromosomal Location of Human Ortholog: Xp22.31 Cellular Component: extracellular region Molecular Function:chemoattractant activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor binding Biological Process: induction of positive chemotaxis; platelet degranulation; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011] |
| UniProt Code: | O43915 |
| NCBI GenInfo Identifier: | 17367984 |
| NCBI Gene ID: | 2277 |
| NCBI Accession: | O43915.1 |
| UniProt Secondary Accession: | O43915,B2R7Z3, |
| UniProt Related Accession: | O43915 |
| Molecular Weight: | 40,444 Da |
| NCBI Full Name: | Vascular endothelial growth factor D |
| NCBI Synonym Full Names: | c-fos induced growth factor |
| NCBI Official Symbol: | FIGF�� |
| NCBI Official Synonym Symbols: | VEGFD; VEGF-D�� |
| NCBI Protein Information: | vascular endothelial growth factor D |
| UniProt Protein Name: | Vascular endothelial growth factor D |
| UniProt Synonym Protein Names: | c-Fos-induced growth factor; FIGF |
| UniProt Gene Name: | FIGF�� |
| UniProt Entry Name: | VEGFD_HUMAN |
