Human VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit
The Human VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit is a powerful tool for accurately measuring levels of VEGF-C in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.VEGF-C is a key protein involved in the regulation of lymphangiogenesis and angiogenesis processes, playing a crucial role in the formation of lymphatic vessels and blood vessels.
Dysregulation of VEGF-C has been linked to various diseases such as cancer, lymphedema, and inflammatory disorders, making it a valuable biomarker for understanding these conditions and developing effective treatments.With its reliable performance and versatility, the Human VEGF-C ELISA Kit is an essential tool for researchers studying vascular biology, cancer biology, and other related fields. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit
SKU:
HUES02609
Target:
Human VEGF-C (Vascular Endothelial Growth Factor C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
65.63 pg/mL
Detection range:
109.38-7000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-C. You can calculate the concentration of Human VEGF-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
92-107
97-112
Average (%)
98
98
105
1:4
Range (%)
99-117
84-96
92-107
Average (%)
107
89
99
1:8
Range (%)
100-112
82-93
96-108
Average (%)
106
87
101
1:16
Range (%)
94-109
82-94
98-113
Average (%)
101
88
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
340.8
586.2
3322.2
340.1
542.6
3620.1
Standard deviation
21.1
31.7
136.2
22.4
25.0
159.3
C V (%)
6.19
5.41
4.1
6.59
4.61
4.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-C in samples. No significant cross-reactivity or interference between Human VEGF-C and analogues was observed.
