Description
| Product Name: | Human VCAM1 Recombinant Protein |
| Product Code: | RPPB5118 |
| Size: | 10µg |
| Species: | Human |
| Target: | VCAM1 |
| Synonyms: | Vascular Cell Adhesion Molecule 1, CD106 Antigen,INCAM-100 , Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, L1CAM. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | VCAM1 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPEFFKIET TPESRYLAQI GDSVSLTCSTTGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDPEIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDIGKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWSKKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGDSVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVELYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMTFIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGFPAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFPSESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLRSLTLDVQGRE NNKDYFSPEH HHHHH |
VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).
VCAM1 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 685 amino acids (25-698 a.a.) andhaving a molecular mass of 75.6kDa (Molecular size on SDS-PAGE will appear atapproximately 70-100kDa). VCAM1 is expressed with a 11 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 1p32-p31 Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane Molecular Function:amine oxidase activity; integrin binding; cell adhesion molecule binding Biological Process: response to nicotine; regulation of immune response; chronic inflammatory response; extracellular matrix organization and biogenesis; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging |
| NCBI Summary: | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | P19320 |
| NCBI GenInfo Identifier: | 137560 |
| NCBI Gene ID: | 7412 |
| NCBI Accession: | P19320.1 |
| UniProt Secondary Accession: | P19320,Q6NUP8, A8K6R7, B4DKS4, E9PDD1, |
| UniProt Related Accession: | P19320 |
| Molecular Weight: | 74,346 Da |
| NCBI Full Name: | Vascular cell adhesion protein 1 |
| NCBI Synonym Full Names: | vascular cell adhesion molecule 1 |
| NCBI Official Symbol: | VCAM1�� |
| NCBI Official Synonym Symbols: | CD106; INCAM-100�� |
| NCBI Protein Information: | vascular cell adhesion protein 1; CD106 antigen |
| UniProt Protein Name: | Vascular cell adhesion protein 1 |
| UniProt Synonym Protein Names: | INCAM-100; CD_antigen: CD106 |
| Protein Family: | Vascular cell adhesion protein |
| UniProt Gene Name: | VCAM1�� |
| UniProt Entry Name: | VCAM1_HUMAN |
