Description
| Product Name: | Human Vaspin Recombinant Protein |
| Product Code: | RPPB1060 |
| Size: | 25µg |
| Species: | Human |
| Target: | Vaspin |
| Synonyms: | Serpin A12 precursor, Visceral adipose-specific serpin, Visceral adipose tissue- derived serine protease inhibitor, Vaspin, OL-64, SERPINA12, Serine (or cysteine) proteinase inhibitor, clade A, antitrypsin, alpha-1 antiproteinase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2?m filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl and 0.02 % Tween-20. |
| Solubility: | It is recommended to reconstitute the lyophilized Vaspin in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.� |
| Stability: | Lyophilized Vaspin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vaspin should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | LKPSFSPRNY KALSEVQGWK QRMAAKELAR QNMDLGFKLL KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ GFNFRKMPEK DLHEGFHYII HELTQKTQDL KLSIGNTLFI DQRLQPQRKF LEDAKNFYSA ETILTNFQNL EMAQKQINDF ISQKTHGKIN NLIENIDPGT VMLLANYIFF RARWKHEFDP NVTKEEDFFL EKNSSVKVPM MFRSGIYQVG YDDKLSCTIL EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK IAPHRSLKVG EAVHKAELKM DERGTEGAAG TGAQTLPMET PLVVKIDKPY LLLIYSEKIP SVLFLGKIVN PIGK |
Vaspin (visceral adipose-specific SERPIN) is a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.
Vaspin Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 394 amino acids and having a molecular mass of 45.1kDa. Vaspin is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family. |
| UniProt Protein Details: | Protein type:Inhibitor; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 14q32.13 Cellular Component: extracellular space; plasma membrane Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway |
| UniProt Code: | Q8IW75 |
| NCBI GenInfo Identifier: | 74728144 |
| NCBI Gene ID: | 145264 |
| NCBI Accession: | Q8IW75.1 |
| UniProt Related Accession: | Q8IW75 |
| Molecular Weight: | 47,175 Da |
| NCBI Full Name: | Serpin A12 |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 |
| NCBI Official Symbol: | SERPINA12�� |
| NCBI Official Synonym Symbols: | OL-64�� |
| NCBI Protein Information: | serpin A12; vaspin; visceral adipose-specific SERPIN; visceral adipose tissue-derived serine protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 |
| UniProt Protein Name: | Serpin A12 |
| UniProt Synonym Protein Names: | OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin |
| Protein Family: | Serpin |
| UniProt Gene Name: | SERPINA12�� |
| UniProt Entry Name: | SPA12_HUMAN |
