Description
| Product Name: | Human VAMP7 Recombinant Protein |
| Product Code: | RPPB5108 |
| Size: | 20µg |
| Species: | Human |
| Target: | VAMP7 |
| Synonyms: | Vesicle-associated membrane protein 7, Tetanus-insensitive VAMP, tetanus neurotoxin-insensitive VAMP, Synaptobrevin-like protein 1, TI-VAMP, VAMP-7, TIVAMP, SYBL1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The VAMP7 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAILFAV VARGTTILAK HAWCGGNFLE VTEQILAKIP SENNKLTYSH GNYLFHYICQ DRIVYLCITD DDFERSRAFN FLNEIKKRFQ TTYGSRAQTA LPYAMNSEFS SVLAAQLKHH SENKGLDKVM ETQAQVDELK GIMVRNIDLV AQRGERLELL IDKTENLVDS SVTFKTTSRN LARAMCMKNL K |
Vesicle-Associated Membrane Protein 7 (VAMP7) is a member of the synaptobrevin family. VAMP7 is a transmembrane protein, which is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. VAMP7 is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. VAMP7 is essential for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is also necessary for calcium regulated lysosomal exocytosis. In addition, VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Furthermore, VAMP7 is needed for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells.
VAMP7 Human Recombinant produced in E. coli is a single polypeptide chain containing 211 amino acids (1-188) and having a molecular mass of 23.0 kDa.VAMP7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | VAMP7: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: Xq28 and Yq12Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; neuron projection; cell surface; intracellular membrane-bound organelle; late endosome membrane; secretory granule membrane; lysosomal membrane; integral to membrane; trans-Golgi network; phagocytic vesicle; pseudopodium; secretory granule; SNARE complex; phagocytic vesicle membrane; membrane; apical part of cell; perinuclear region of cytoplasm; lamellipodium; cytoplasm; plasma membrane; synapse; cell junctionMolecular Function: SNAP receptor activity; protein binding; SNARE binding; syntaxin bindingBiological Process: ER to Golgi vesicle-mediated transport; exocytosis; natural killer cell degranulation; autophagic vacuole fusion; neutrophil degranulation; endocytosis; vesicle fusion with Golgi apparatus; calcium ion-dependent exocytosis; vesicle fusion; vesicle-mediated transport; Golgi to plasma membrane protein transport; eosinophil degranulation; endosome to lysosome transport; post-Golgi vesicle-mediated transport; triacylglycerol transport; phagocytosis, engulfment |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010] |
| UniProt Code: | P51809 |
| NCBI GenInfo Identifier: | 1723133 |
| NCBI Gene ID: | 6845 |
| NCBI Accession: | P51809.3 |
| UniProt Secondary Accession: | P51809,Q53GY7, Q7Z409, Q9H4A7 |
| UniProt Related Accession: | P51809 |
| Molecular Weight: | 20,121 Da |
| NCBI Full Name: | Vesicle-associated membrane protein 7 |
| NCBI Synonym Full Names: | vesicle-associated membrane protein 7 |
| NCBI Official Symbol: | VAMP7�� |
| NCBI Official Synonym Symbols: | SYBL1; TIVAMP; VAMP-7; TI-VAMP�� |
| NCBI Protein Information: | vesicle-associated membrane protein 7; synaptobrevin-like 1; tetanus-insensitive VAMP; synaptobrevin-like protein 1; tetanus neurotoxin-insensitive VAMP |
| UniProt Protein Name: | Vesicle-associated membrane protein 7 |
| UniProt Synonym Protein Names: | Synaptobrevin-like protein 1; Tetanus-insensitive VAMP; Ti-VAMP |
| Protein Family: | Vesicle-associated membrane protein |
| UniProt Gene Name: | VAMP7�� |
| UniProt Entry Name: | VAMP7_HUMAN |
