The Human UST2 (Urotensin 2) ELISA Kit is specifically designed for the precise and sensitive detection of Urotensin 2 levels in human serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and reproducibility, making it an invaluable tool for a wide range of research applications.Urotensin 2 is a critical peptide involved in regulating blood pressure and cardiovascular function, and has been implicated in various cardiovascular diseases.
It serves as a key biomarker for studying these conditions and developing potential therapeutic interventions. Don't miss out on the opportunity to advance your research with the Human UST2 ELISA Kit from Assay Genie.
Product Name:
Human UST2 (Urotensin 2) ELISA Kit
SKU:
HUES02966
Target:
Human UST2 (Urotensin 2)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with UST2. During the reaction, UST2 in the sample or standard competes with a fixed amount of UST2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to UST2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UST2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
98-110
91-104
Average (%)
96
104
97
1:4
Range (%)
88-100
90-101
94-109
Average (%)
94
96
100
1:8
Range (%)
91-102
86-102
101-114
Average (%)
96
93
107
1:16
Range (%)
90-103
87-100
99-114
Average (%)
96
92
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
3.1
8.9
1.0
3.1
9.0
Standard deviation
0.1
0.2
0.4
0.1
0.1
0.5
C V (%)
10
6.45
4.49
10.0
3.23
5.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of UST2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes UST2 in samples. No significant cross-reactivity or interference between UST2 and analogues was observed.
