Description
| Product Name: | Human Ubiquitin K48R Recombinant Protein |
| Product Code: | RPPB5066 |
| Size: | 20µg |
| Species: | Human |
| Target: | Ubiquitin K48R |
| Synonyms: | Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin K48R. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | Diluted in PBS plus 5% glycerol. |
| Stability: | Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
Ubiquitin is a highly conserved 76 amino acid protein expressed in all eukariotes. Ub is found either in free form or conjugated to proteins as monomer or as chain of ubiquitin molecules. The most well characterized consequence of polyubiquitination is substrate degradation, while mono-ubiquitinated proteins are not degraded. Ubiquitin attachment to protein substrate is a complex process involving a ubiquitin activating enzyme (E1), a ubiquitin conjugating enzyme (E2) and a ubiquitin protein ligase (E3). The first ubiquitin moiety is transferred to the e-NH2 group of a Lys residue of the protein substrate to generate an isopeptide bond. In successive reactions, a poly ubiquitin chain is synthesized by processive transfer of additional activated moieties to Lys48 of the previously conjugated ubiquitin molecule. Ubiquitin K48R prevents the formation of poly ubiquitin chains via Lys48 linkages with mono ubiquitin molecules, avoiding the degradation of protein substrates.
Recombinant human ubiquitin featuring a Lys 48 to Arg48 mutation is useful for the reduction of poly-Ub chain length and conjugation rates.Ubiquitin K48R is expressed in E.coliand purified by proprietary chromatographic techniques.
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62988 |
| NCBI GenInfo Identifier: | 51703339 |
| NCBI Gene ID: | 7311 |
| NCBI Accession: | P62988.1 |
| UniProt Secondary Accession: | P62988 |
| UniProt Related Accession: | P62988,P62987 |
| Molecular Weight: | |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | ubiquitin A-52 residue ribosomal protein fusion product 1 |
| NCBI Official Symbol: | UBA52�� |
| NCBI Official Synonym Symbols: | L40; CEP52; RPL40; HUBCEP52�� |
| NCBI Protein Information: | ubiquitin-60S ribosomal protein L40 |
| UniProt Protein Name: | |
| UniProt Synonym Protein Names: | |
| Protein Family: | Ubiquitin |
| UniProt Gene Name: | |
| UniProt Entry Name: |
