Description
| Product Name: | Human UBE2W Recombinant Protein |
| Product Code: | RPPB2393 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2W |
| Synonyms: | Probable ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-protein ligase W, UBE2W, hUBC-16, FLJ11011. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | UBE2W solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| Stability: | UBE2W Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C |
UBE2W receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2W catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is broadly expressed, particularly at highest levels in the testis.
UBE2W produced in E.Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.5kDa.UBE2W is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ligase Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: misfolded or incompletely synthesized protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination |
| NCBI Summary: | This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| UniProt Code: | Q96B02 |
| NCBI GenInfo Identifier: | 74751754 |
| NCBI Gene ID: | 55284 |
| NCBI Accession: | Q96B02.1 |
| UniProt Secondary Accession: | Q96B02,Q1XBE0, Q9H823, Q9HAG6, Q9NV07, B4DIV1, |
| UniProt Related Accession: | Q96B02 |
| Molecular Weight: | 20,689 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 W |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 W (putative) |
| NCBI Official Symbol: | UBE2W�� |
| NCBI Official Synonym Symbols: | UBC16; UBC-16�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 W |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 W |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme W; N-terminal E2 ubiquitin-conjugating enzyme (EC:2.3.2.25 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2W�� |
| UniProt Entry Name: | UBE2W_HUMAN |
