Description
| Product Name: | Human TYW5 Recombinant Protein |
| Product Code: | RPPB5057 |
| Size: | 20µg |
| Species: | Human |
| Target: | TYW5 |
| Synonyms: | tRNA wybutosine-synthesizing protein 5, hTYW5, TYW5, C2orf60. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TYW5 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE |
tRNA wybutosine-synthesizing protein 5 (TYW5) functions as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position closest to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine observed in higher eukaryotes.
TYW5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-315 a.a.) and having a molecular mass of 38.9kDa.TYW5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TYW5: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes. Belongs to the TYW5 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 1.14.11.42Chromosomal Location of Human Ortholog: 2q33.1Molecular Function: protein homodimerization activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA bindingBiological Process: wybutosine biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | A2RUC4 |
| NCBI GenInfo Identifier: | 89242134 |
| NCBI Gene ID: | 129450 |
| NCBI Accession: | NP_001034782 |
| UniProt Secondary Accession: | A2RUC4 |
| UniProt Related Accession: | A2RUC4 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | tRNA wybutosine-synthesizing protein 5 |
| NCBI Synonym Full Names: | tRNA-yW synthesizing protein 5 |
| NCBI Official Symbol: | TYW5�� |
| NCBI Official Synonym Symbols: | hTYW5; C2orf60�� |
| NCBI Protein Information: | tRNA wybutosine-synthesizing protein 5 |
| UniProt Protein Name: | tRNA wybutosine-synthesizing protein 5 |
| UniProt Synonym Protein Names: | tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase |
| Protein Family: | tRNA wybutosine-synthesizing protein |
| UniProt Gene Name: | TYW5�� |
| UniProt Entry Name: | TYW5_HUMAN |
