Description
| Product Name: | Human TUBB3 Recombinant Protein |
| Product Code: | RPPB5038 |
| Size: | 10µg |
| Species: | Human |
| Target: | TUBB3 |
| Synonyms: | Beta-4, CDCBM, CFEOM3A, TUBB4 , Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TUBB3 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMREIVHI QAGQCGNQIG AKFWEVISDE HGIDPSGNYV GDSDLQLERI SVYYNEASSH KYVPRAILVD LEPGTMDSVR SGAFGHLFRP DNFIFGQSGA GNNWAKGHYT EGAELVDSVL DVVRKECENC DCLQGFQLTH SLGGGTGSGM GTLLISKVRE EYPDRIMNTF SVVPSPKVSD TVVEPYNATL SIHQLVENTD ETYCIDNEAL YDICFRTLKL ATPTYGDLNH LVSATMSGVT TSLRFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTARG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVATVF RGRMSMKEVD EQMLAIQSKN SSYFVEWIPN NVKVAVCDIP PRGLKMSSTF IGNSTAIQEL FKRISEQFTA MFRRKAFLHW YTGEGMDEME FTEAESNMND LVSEYQQYQD ATAEEEGEMY EDDEEESEAQ GPK |
Tubulin, beta 3 class III (TUBB3), which is the major constituent of microtubules, is primarily Expressed in neurons and is involved in neurogenesis and in axon guidance and maintenance. TUBB3 binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
TUBB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 473 amino acids (1-450 a.a.) and having a molecular mass of 52.8kDa. TUBB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 16q24.3 Cellular Component: axon; cell soma; cytoplasm; dendrite; microtubule; nucleus Molecular Function:GTP binding; GTPase activity; peptide binding; protein binding; structural constituent of cytoskeleton Biological Process: 'de novo' posttranslational protein folding; axon guidance; cellular protein metabolic process; microtubule-based process; mitosis; protein folding Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 1; Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement |
| NCBI Summary: | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q13509 |
| NCBI GenInfo Identifier: | 20455526 |
| NCBI Gene ID: | 10381 |
| NCBI Accession: | Q13509.2 |
| UniProt Secondary Accession: | Q13509,Q9BTZ0, Q9BW10, A8K854, |
| UniProt Related Accession: | Q13509 |
| Molecular Weight: | 42,433 Da |
| NCBI Full Name: | Tubulin beta-3 chain |
| NCBI Synonym Full Names: | tubulin beta 3 class III |
| NCBI Official Symbol: | TUBB3�� |
| NCBI Official Synonym Symbols: | CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A�� |
| NCBI Protein Information: | tubulin beta-3 chain |
| UniProt Protein Name: | Tubulin beta-3 chain |
| UniProt Synonym Protein Names: | Tubulin beta-4 chain; Tubulin beta-III |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBB3�� |
| UniProt Entry Name: | TBB3_HUMAN |
| Amir Niaraki et al. | Minute-sensitive real-time monitoring of neural cells through printed graphene microelectrodes | Biosensors and Bioelectronics 2022 | PubMed ID: 35462297 |
