The Human TUB-beta (Beta-Tubulin) CLIA Kit is a comprehensive solution for quantifying beta-tubulin levels in human samples, including serum, plasma, and cell culture supernatants. This high-performance kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Beta-tubulin is a key component of microtubules, essential for cell structure, division, and transport processes. Dysregulation of beta-tubulin has been linked to various diseases such as cancer and neurological disorders, underscoring its importance as a biomarker for disease progression and therapeutic development.
With the Human TUB-beta CLIA Kit, researchers can confidently study beta-tubulin levels in different biological samples, gaining valuable insights into disease mechanisms and potential treatment strategies. Trust in the precision and reliability of this kit for your research needs.
Product Name:
Human TUB beta (beta-Tubulin) CLIA Kit
SKU:
HUES00594
Target:
Human TUB beta (beta-Tubulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TUBβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TUBβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TUBβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TUBβ. You can calculate the concentration of Human TUBβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-117
90-102
101-117
Average (%)
110
96
109
1:4
Range (%)
93-104
90-101
97-111
Average (%)
99
96
105
1:8
Range (%)
97-114
102-116
83-95
Average (%)
104
108
90
1:16
Range (%)
103-118
94-110
103-119
Average (%)
108
100
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.17
311.02
943.64
105.54
295.71
1021.27
Standard deviation
10.09
33.22
100.21
13.42
26.14
82.42
C V (%)
9.16
10.68
10.62
12.72
8.84
8.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TUBβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TUBβ in samples. No significant cross-reactivity or interference between Human TUBβ and analogues was observed.
