Description
| Product Name: | Human Troponin-C Recombinant Protein |
| Product Code: | RPPB5015 |
| Size: | 10µg |
| Species: | Human |
| Target: | Troponin-C |
| Synonyms: | Troponin C slow skeletal and cardiac muscles, TN-C, TNNC1, TNNC, TNC, CMD1Z. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The Cardiac Troponin-C solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GKSEEELSDL FRMFDKNADG YIDLDELKIM LQATGETITE DDIEELMKDG DKNNDGRIDY DEFLEFMKGV E |
Troponin is a fundamental regulatory protein of striated muscle contraction, and together with tropomyosin, is positioned on the actin filament. Troponin has 3 subunits: TnI- the inhibitor of actomyosin ATPase; TnT- contains the binding site for tropomyosin; and TnC- the protein encoded by the TNNC1 gene. The binding of calcium to TnC stops the inhibitory action of TnI, consequently allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in the TNNC1 gene are linked to cardiomyopathy dilated type 1Z.
Cardiac Troponin-C Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (1-161) and having a molecular mass of 20.5 kDa.Cardiac Troponin-C is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family. |
| UniProt Protein Details: | Protein type:Calcium-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: nucleoplasm; troponin complex; mitochondrion; cytosol; actin cytoskeleton Molecular Function:troponin T binding; actin filament binding; protein binding; protein homodimerization activity; troponin I binding; calcium ion binding; calcium-dependent protein binding Biological Process: response to metal ion; regulation of ATPase activity; regulation of muscle contraction; diaphragm contraction; ventricular cardiac muscle morphogenesis; regulation of muscle filament sliding speed; cardiac muscle contraction; muscle filament sliding Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13 |
| NCBI Summary: | Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P63316 |
| NCBI GenInfo Identifier: | 54042075 |
| NCBI Gene ID: | 7134 |
| NCBI Accession: | P63316.1 |
| UniProt Related Accession: | P63316 |
| Molecular Weight: | |
| NCBI Full Name: | Troponin C, slow skeletal and cardiac muscles |
| NCBI Synonym Full Names: | troponin C1, slow skeletal and cardiac type |
| NCBI Official Symbol: | TNNC1�� |
| NCBI Official Synonym Symbols: | TNC; TN-C; TNNC; CMD1Z; CMH13�� |
| NCBI Protein Information: | troponin C, slow skeletal and cardiac muscles |
| UniProt Protein Name: | Troponin C, slow skeletal and cardiac muscles |
| Protein Family: | Tenascin |
| UniProt Gene Name: | TNNC1�� |
| UniProt Entry Name: | TNNC1_HUMAN |
