Description
| Product Name: | Human TRIM33 Recombinant Protein |
| Product Code: | RPPB5009 |
| Size: | 10µg |
| Species: | Human |
| Target: | TRIM33 |
| Synonyms: | E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Protein Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, Tripartite motif-containing protein 33, TRIM33, KIAA1113, RFG7, TIF1G, ECTO, PTC7, TF1G, TIFGAMMA, TIF1GAMMA. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TRIM33 is supplied in 20mM HEPES buffer pH-7.9, 550mM NaCl and 6M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
Tripartite Motif Containing 33 (TRIM33) belongs to the tripartite motif family. This motif includes 3 zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM33 is assumed to be a transcriptional corepressor. TRIM33 functions as an E3 ubiquitin-protein ligase. TRIM33 also promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. In addition, TRIM33 inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Furthermore, TRIM33 has a role in the control of cell proliferation.
TRIM33 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 124,041 Dalton. TRIM33 is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Kinase, protein; Ubiquitin ligase; Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Transcription, coactivator/corepressor; EC 6.3.2.-; Protein kinase, atypical; ATYPICAL group; TIF1 family Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: negative regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; protein ubiquitination; regulation of transforming growth factor beta receptor signaling pathway Disease: Thyroid Carcinoma, Papillary |
| NCBI Summary: | The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UPN9 |
| NCBI GenInfo Identifier: | 313104270 |
| NCBI Gene ID: | 51592 |
| NCBI Accession: | Q9UPN9.3 |
| UniProt Secondary Accession: | Q9UPN9,O95855, Q5TG72, Q5TG73, Q5TG74, Q9C017, Q9UJ79 |
| UniProt Related Accession: | Q9UPN9 |
| Molecular Weight: | 120,541 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase TRIM33 |
| NCBI Synonym Full Names: | tripartite motif containing 33 |
| NCBI Official Symbol: | TRIM33�� |
| NCBI Official Synonym Symbols: | ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA�� |
| NCBI Protein Information: | E3 ubiquitin-protein ligase TRIM33 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase TRIM33 |
| UniProt Synonym Protein Names: | Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | TRIM33�� |
| UniProt Entry Name: | TRI33_HUMAN |
