Description
| Product Name: | Human TNFSF12 Recombinant Protein |
| Product Code: | RPPB1030 |
| Size: | 25µg |
| Species: | Human |
| Target: | TNFSF12 |
| Synonyms: | TWEAK, TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand, APO3L, TNFRSF12A, Tumor necrosis factor ligand superfamily member 12, MGC20669, MGC129581. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2?m filtered solution in PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH |
| Biological Activity: | The ED50, as measured in a proliferation assay using HUVECs, is less than 8ng/ml. |
TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion.
TNFSF12 Human Recombinant (94-249 a.a.) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a total molecular mass of 18kDa.The TNFSF12 is fused with an 8 amino acids his tag at N-terminal (M-HHHHHH-R, total 164 a.a.) and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family. |
| UniProt Protein Details: | Protein type:Apoptosis; Membrane protein, integral; Motility/polarity/chemotaxis; Cytokine Chromosomal Location of Human Ortholog: 17p13 Cellular Component: extracellular space; perinuclear region of cytoplasm; integral to plasma membrane Molecular Function:protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction |
| NCBI Summary: | This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43508 |
| NCBI GenInfo Identifier: | 21362987 |
| NCBI Gene ID: | 407977 |
| NCBI Accession: | O43508.1 |
| UniProt Secondary Accession: | O43508,Q8IZK7, Q8WUZ7, |
| Molecular Weight: | 36,589 Da |
| NCBI Full Name: | Tumor necrosis factor ligand superfamily member 12 |
| NCBI Synonym Full Names: | TNFSF12-TNFSF13 readthrough |
| NCBI Official Symbol: | TNFSF12-TNFSF13�� |
| NCBI Official Synonym Symbols: | TWE-PRIL�� |
| NCBI Protein Information: | TNFSF12-TNFSF13 protein; tumor necrosis factor (ligand) superfamily, member 12-member 13 |
| UniProt Protein Name: | Tumor necrosis factor ligand superfamily member 12 |
| UniProt Synonym Protein Names: | APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK |
| UniProt Gene Name: | TNFSF12�� |
| UniProt Entry Name: | TNF12_HUMAN |
