Description
| Product Name: | Human TNFRSF12A Recombinant Protein |
| Product Code: | RPPB1031 |
| Size: | 25µg |
| Species: | Human |
| Target: | TNFRSF12A |
| Synonyms: | Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized TNFRSF12A in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TNFRSF12A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF12A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP |
| Biological Activity: | The TNFRSF12A biological activity is determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-5.0ug/ml in the presence of 1ug/ml rhTWEAK. |
The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.
TNFRSF12A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 5.6 KDa.The TNFRSF12A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc.; Cell development/differentiation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: ruffle; cell surface; plasma membrane; integral to membrane Molecular Function:protein binding Biological Process: substrate-bound cell migration, cell attachment to substrate; positive regulation of apoptosis; positive regulation of axon extension; angiogenesis; regulation of angiogenesis |
| UniProt Code: | Q9NP84 |
| NCBI GenInfo Identifier: | 21263626 |
| NCBI Gene ID: | 51330 |
| NCBI Accession: | Q9NP84.1 |
| UniProt Secondary Accession: | Q9NP84,Q9HCS0, D3DUA6, |
| UniProt Related Accession: | Q9NP84 |
| Molecular Weight: | 10,191 Da |
| NCBI Full Name: | Tumor necrosis factor receptor superfamily member 12A |
| NCBI Synonym Full Names: | tumor necrosis factor receptor superfamily, member 12A |
| NCBI Official Symbol: | TNFRSF12A�� |
| NCBI Official Synonym Symbols: | FN14; CD266; TWEAKR�� |
| NCBI Protein Information: | tumor necrosis factor receptor superfamily member 12A; tweak-receptor; FGF-inducible 14; type I transmembrane protein Fn14; fibroblast growth factor-inducible immediate-early response protein 14 |
| UniProt Protein Name: | Tumor necrosis factor receptor superfamily member 12A |
| UniProt Synonym Protein Names: | Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD_antigen: CD266 |
| UniProt Gene Name: | TNFRSF12A�� |
| UniProt Entry Name: | TNR12_HUMAN |
