Description
| Product Name: | Human TIGIT Recombinant Protein |
| Product Code: | RPPB4961 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIGIT |
| Synonyms: | VSIG9, VSTM3, WUCAM, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TIGIT protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MMTGTIETTG NISAEKGGSI ILQCHLSSTT AQVTQVNWEQ QDQLLAICNA DLGWHISPSF KDRVAPGPGL GLTLQSLTVN DTGEYFCIYH TYPDGTYTGR IFLEVLESSV AEHGARFQIP LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK�FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH |
T cell immunoreceptor with Ig and ITIM domains or TIGIT, is an immune receptor located on part of T cells and Natural Killer Cells(NK). TIGIT is also known as Vstm3 and WUCAM. The receptor can bind with high affinity to dendritic cells(DCs), CD155(PVR) on macrophages, etc., and it can bind with lower affinity to CD112(PVRL2). TIGIT can inhibit the cytotoxicity of Natural Killer Cells through its ITIM domain via antibodies against the interaction with PVR and the activity is directed. It has been show on research that TIGIT-Fc fusion molecule can have interaction with PVR on dendritic cells and elevate its IL-10 secretion level or lower the secretion on IL-12 under the stimulation of LPS, moreover, it can inhibit the activation of T cell in vivo.
TIGIT produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 359 amino acids (22-141a.a.) and having a molecular mass of 40.0kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).TIGIT is expressed with a 239 amino acid hlgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009] |
| UniProt Code: | Q495A1 |
| NCBI GenInfo Identifier: | 121943253 |
| NCBI Gene ID: | 201633 |
| NCBI Accession: | Q495A1.1 |
| UniProt Secondary Accession: | Q495A1,Q495A3, Q5JPD8, Q6MZS2, Q8N877 |
| UniProt Related Accession: | Q495A1 |
| Molecular Weight: | 18,432 Da |
| NCBI Full Name: | T-cell immunoreceptor with Ig and ITIM domains |
| NCBI Synonym Full Names: | T cell immunoreceptor with Ig and ITIM domains |
| NCBI Official Symbol: | TIGIT�� |
| NCBI Official Synonym Symbols: | VSIG9; VSTM3; WUCAM�� |
| NCBI Protein Information: | T-cell immunoreceptor with Ig and ITIM domains |
| UniProt Protein Name: | T-cell immunoreceptor with Ig and ITIM domains |
| UniProt Synonym Protein Names: | V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3 |
| Protein Family: | T-cell immunoreceptor with Ig and ITIM domains |
| UniProt Gene Name: | TIGIT�� |
| UniProt Entry Name: |
