Description
| Product Name: | Human TCEB1 Recombinant Protein |
| Product Code: | RPPB4926 |
| Size: | 20µg |
| Species: | Human |
| Target: | TCEB1 |
| Synonyms: | Transcription elongation factor B polypeptide 1, Elongin 15 kDa subunit, Elongin-C, EloC, RNA polymerase II transcription factor SIII subunit C, SIII p15, TCEB1, SIII. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TCEB1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.15M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC |
TCEB1 (elongin C) is a subunit of elongin, which is a common transcription elongation factor that increases the RNA polymerase II transcription elongation past template encoded arresting sites. The SIII complex is comprised of elongins A/A2, B and C. The SIII complex activates elongation by RNA polymerase II by curbing transient pausing of the polymerase at numerous sites within transcription units. Elongin A acts as the transcriptionally active component of the SIII complex, while elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and able to forming a stable complex with elongins B and C. The VHL (von Hippel-Lindau) tumor suppressor protein binds to elongins B and C, and thus inhibits transcription elongation.
TCEB1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-112 a.a.) and having a molecular mass of 14.6kDa. TCEB1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TCEB1: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Belongs to the SKP1 family. |
| UniProt Protein Details: | Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: cytosol; nucleoplasm Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: positive regulation of RNA elongation from RNA polymerase II promoter; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q15369 |
| NCBI GenInfo Identifier: | 32699511 |
| NCBI Gene ID: | 6921 |
| NCBI Accession: | Q15369.1 |
| UniProt Secondary Accession: | Q15369,Q567Q6, E5RGD9, |
| UniProt Related Accession: | Q15369 |
| Molecular Weight: | 10,832 Da |
| NCBI Full Name: | Transcription elongation factor B polypeptide 1 |
| NCBI Synonym Full Names: | transcription elongation factor B subunit 1 |
| NCBI Official Symbol: | TCEB1�� |
| NCBI Official Synonym Symbols: | SIII; eloC�� |
| NCBI Protein Information: | transcription elongation factor B polypeptide 1 |
| UniProt Protein Name: | Transcription elongation factor B polypeptide 1 |
| UniProt Synonym Protein Names: | Elongin 15 kDa subunit; Elongin-C; EloC; RNA polymerase II transcription factor SIII subunit C; SIII p15 |
| Protein Family: | Transcription elongation factor B polypeptide |
| UniProt Gene Name: | TCEB1�� |
| UniProt Entry Name: | ELOC_HUMAN |
