Description
| Product Name: | Human TBCC Recombinant Protein |
| Product Code: | RPPB4917 |
| Size: | 10µg |
| Species: | Human |
| Target: | TBCC |
| Synonyms: | Tubulin folding cofactor C, tubulin-specific chaperone c, Tubulin-folding cofactor C, CFC. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TBCC solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMESVSCS AAAVRTGDME SQRDLSLVPE RLQRREQERQ LEVERRKQKR QNQEVEKENS HFFVATFARE RAAVEELLER AESVERLEEA ASRLQGLQKL INDSVFFLAA YDLRQGQEAL ARLQAALAER RRGLQPKKRF AFKTRGKDAA SSTKVDAAPG IPPAVESIQD SPLPKKAEGD LGPSWVCGFS NLESQVLEKR ASELHQRDVL LTELSNCTVR LYGNPNTLRL TKAHSCKLLC GPVSTSVFLE DCSDCVLAVA CQQLRIHSTK DTRIFLQVTS RAIVEDCSGI QFAPYTWSYP EIDKDFESSG LDRSKNNWND VDDFNWLARD MASPNWSILP EEERNIQWD |
Tubulin folding cofactor C (TBCC) is a member of the TBCC family. TBCC has a role in the control of centrosome and Golgi apparatus positioning, with effects on cell shape and cell migration. Cofactor C is 1 of 4 proteins (cofactors A,D,E and C) engaged in the pathway leading to properly folded b-tubulin from folding intermediates. Cofactor E attaches to the cofactor D/beta-tubulin complex; their interaction with cofactor C subsequently causes the release of beta-tubulin polypeptides which are bound to the native state.
TBCC Human Recombinant produced in E. coli is a single polypeptide chain containing 369 amino acids (1-346) and having a molecular mass of 41.7 kDa.TBCC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TBCC: Tubulin-folding protein; involved in the final step of the tubulin folding pathway. Belongs to the TBCC family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: cytoplasm; cytoskeleton; microtubule; photoreceptor connecting cilium Molecular Function:chaperone binding; GTPase activity Biological Process: post-chaperonin tubulin folding pathway; protein folding; tubulin folding |
| NCBI Summary: | Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15814 |
| NCBI GenInfo Identifier: | 313104020 |
| NCBI Gene ID: | 6903 |
| NCBI Accession: | Q15814.2 |
| UniProt Secondary Accession: | Q15814,Q53Y43, Q5T787, |
| UniProt Related Accession: | Q15814 |
| Molecular Weight: | 39,248 Da |
| NCBI Full Name: | Tubulin-specific chaperone C |
| NCBI Synonym Full Names: | tubulin folding cofactor C |
| NCBI Official Symbol: | TBCC�� |
| NCBI Official Synonym Symbols: | CFC�� |
| NCBI Protein Information: | tubulin-specific chaperone C |
| UniProt Protein Name: | Tubulin-specific chaperone C |
| UniProt Synonym Protein Names: | Tubulin-folding cofactor C; CFC |
| Protein Family: | TBCC domain-containing protein |
| UniProt Gene Name: | TBCC�� |
| UniProt Entry Name: | TBCC_HUMAN |
