Description
| Product Name: | Human TARDBP (1-414) Recombinant Protein |
| Product Code: | RPPB4908 |
| Size: | 20µg |
| Species: | Human |
| Target: | TARDBP (1-414) |
| Synonyms: | ALS10,TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TARDBPprotein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glyceroland 0.4M urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM |
TARDNA Binding Protein (TARDBP) was initially recognized as a transcriptional repressorwhich binds to chromosomally integrated TAR DNA and represses HIV-1transcription. In addition, TARDBP has been detected in individuals diagnosedwith chronic traumatic encephalopathy as well, which is a condition that oftenimitates ALS and hasbeen related with athletes who have experienced multiple concussions and othertypes of head injury.TARDBPhas been shown to bind both DNA and RNA, it has multiple functions intranscriptional repression, pre-mRNA splicing and translational regulation.
TARDBP Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 450 amino acids (1-414 a.a) andhaving a total molecular mass of 48.8 kDa. TARDBP is fused to a 36 amino acid His-tag atN-terminus & purified by proprietary chromatographic techniques.
| NCBI Summary: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13148 |
| NCBI GenInfo Identifier: | 20140568 |
| NCBI Gene ID: | 23435 |
| NCBI Accession: | |
| UniProt Related Accession: | Q13148 |
| Molecular Weight: | 44 |
| NCBI Full Name: | TAR DNA-binding protein 43 |
| NCBI Synonym Full Names: | TAR DNA binding protein |
| NCBI Official Symbol: | TARDBP�� |
| NCBI Official Synonym Symbols: | ALS10; TDP-43�� |
| NCBI Protein Information: | TAR DNA-binding protein 43 |
| UniProt Protein Name: | |
| UniProt Gene Name: |
