Description
| Product Name: | Human SUGT1 Recombinant Protein |
| Product Code: | RPPB4874 |
| Size: | 20µg |
| Species: | Human |
| Target: | SUGT1 |
| Synonyms: | SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae), SGT1, Putative 40-6-3 protein, SGT1B protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The SGT1 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MHRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQVVITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADVKNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWKKY |
SUGT1 is homolog to yeast protein SGT1, a regulator of the cell cycle which is vital for G1/S and G2/M transitions. SUGT1 holds a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR). SUGT1 protein associates with Skp1 p19 and CUL-1, subunits of the SCF ubiquitin ligase complex, and is believed to take a part in protein degradation. Furthermore, SUGT1 is essential for the kinetochores assembly, and has a role as a co-chaperone for HSP90.
SGT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (115-365.a.a) and having a molecular mass of 30.7kDa. SGT1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SUGT1: a protein involved in kinetochore function and required for the G1/S and G2/M transitions. A subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes. Two alternatively spliced isoforms have been reported. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: cytosol; kinetochore; protein complex; ubiquitin ligase complex Molecular Function:protein binding Biological Process: mitosis; regulation of protein stability |
| NCBI Summary: | This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. This protein interacts with heat shock protein 90. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene have been defined on several different chromosomes. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q9Y2Z0 |
| NCBI GenInfo Identifier: | 62512186 |
| NCBI Gene ID: | 10910 |
| NCBI Accession: | Q9Y2Z0.3 |
| UniProt Secondary Accession: | Q9Y2Z0,Q5JAK5, Q5TAM6, Q6VXY6, A2A303, |
| UniProt Related Accession: | Q9Y2Z0 |
| Molecular Weight: | 37,805 Da |
| NCBI Full Name: | Protein SGT1 homolog |
| NCBI Synonym Full Names: | SGT1 homolog, MIS12 kinetochore complex assembly cochaperone |
| NCBI Official Symbol: | SUGT1�� |
| NCBI Official Synonym Symbols: | SGT1�� |
| NCBI Protein Information: | protein SGT1 homolog |
| UniProt Protein Name: | Protein SGT1 homolog |
| UniProt Synonym Protein Names: | Protein 40-6-3 |
| UniProt Gene Name: | SUGT1�� |
| UniProt Entry Name: | SGT1_HUMAN |
