Description
| Product Name: | Human STAMBP Recombinant Protein |
| Product Code: | RPPB4838 |
| Size: | 20µg |
| Species: | Human |
| Target: | STAMBP |
| Synonyms: | STAM-binding protein, Associated molecule with the SH3domain of STAM, Endosome-associated ubiquitin isopeptidase, AMSH, EC 3.1.2.15,EC 3.4.19, MICCAP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | STAMBP protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 85% as determined by SDS-PAGE.. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSDHGDV SLPPEDRVRA LSQLGSAVEV NEDIPPRRYF RSGVEIIRMASIYSEEGNIE HAFILYNKYI TLFIEKLPKH RDYKSAVIPE KKDTVKKLKE IAFPKAEELK AELLKRYTKEYTEYNEEKKK EAEELARNMA IQQELEKEKQ RVAQQKQQQL EQEQFHAFEE MIRNQELEKE RLKIVQEFGKVDPGLGGPLV PDLEKPSLDV FPTLTVSSIQ PSDCHTTVRP AKPPVVDRSL KPGALSNSES IPTIDGLRHVVVPGRLCPQF LQLASANTAR GVETCGILCG KLMRNEFTIT HVLIPKQSAG SDYCNTENEE ELFLIQDQQGLITLGWIHTH PTQTAFLSSV DLHTHCSYQM MLPESVAIVC SPKFQETGFF KLTDHGLEEI SSCRQKGFHP HSKDPPLFCSCSHVTVVDRA VTITDLR |
STAM Binding Protein (STAMBP) is a zinc metalloprotease which specifically cleaves Lys-63-linked polyubiquitin chains, however it doesn�t cleave Lys-48-linked polyubiquitin chains. STAMBP has a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. In addition, STAMBP potentiates BMP signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. STAMBP also has a major role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is essential for efficient EGFR degradation but not for its internalization. STAMBP is also involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
STAMBP Human Recombinant produced in E.coli is a single,non-glycosylated polypeptide chain containing 447 amino acids (1-424) andhaving a molecular mass of 50kDa.�STAMBP is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AMSH: an adaptor protein involved in cytokine signal transduction the JAK-STAT cascade. Binds to the SH3 domain of the signal-transducing adaptor molecule STAM (signal transducing adaptor molecule). May play a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. |
| UniProt Protein Details: | Protein type:EC 3.1.2.15; Protease; Ubiquitin-specific protease; EC 3.4.19.- Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: cleavage furrow; cytoplasm; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding; ubiquitin-specific protease activity Biological Process: cytokinesis after mitosis; JAK-STAT cascade; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of Ras protein signal transduction; positive regulation of cell proliferation; protein deubiquitination Disease: Microcephaly-capillary Malformation Syndrome |
| NCBI Summary: | Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95630 |
| NCBI GenInfo Identifier: | 71153538 |
| NCBI Gene ID: | 10617 |
| NCBI Accession: | O95630.1 |
| UniProt Secondary Accession: | O95630,Q3MJE7, B5M0B6, D6W5H7, |
| UniProt Related Accession: | O95630 |
| Molecular Weight: | 39,596 Da |
| NCBI Full Name: | STAM-binding protein |
| NCBI Synonym Full Names: | STAM binding protein |
| NCBI Official Symbol: | STAMBP�� |
| NCBI Official Synonym Symbols: | AMSH; MICCAP�� |
| NCBI Protein Information: | STAM-binding protein |
| UniProt Protein Name: | STAM-binding protein |
| UniProt Synonym Protein Names: | Associated molecule with the SH3 domain of STAM; Endosome-associated ubiquitin isopeptidase |
| Protein Family: | STAM-binding protein |
| UniProt Gene Name: | STAMBP�� |
| UniProt Entry Name: | STABP_HUMAN |
