Description
| Product Name: | Human SRP54 Recombinant Protein |
| Product Code: | RPPB4827 |
| Size: | 20µg |
| Species: | Human |
| Target: | SRP54 |
| Synonyms: | Signal Recognition Particle 54 kDa protein |
| Source: | Sf9 Insect cells |
| Formulation: | SRP54 is supplied in 16mM HEPES buffer pH-8.0, 320mM NaCl, 5mM DTT, 1mM EDTA, 0.16mM GDP and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80% as determined by SDS-PAGE. |
SRP54 is a 54-kDa subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex which leads the translocation of newly synthesized secretory proteins from the polysome to the endoplasmic reticulum. SRP is composed of six polypeptides and a tRNA-like molecule known as 7SL RNA. SRP54 is a GTP-binding protein that directly binds the signal sequences of nascent secretory and membrane proteins. Anti-SRP autoantibodies take place in patients with an autoimmune chronic muscle inflammation called polymyositis. These auto-antibodies predominantly recognize the SRP54 subunit and in addition are able to immunoprecipitate several of the SRP subunits and the RNA component. Nearly 5% of myositis patients are positive for anti-SRP autoantibodies, increasing to 18% in the subgroup of Jo-1 autoantibody-negative patients. The classic 'anti-SRP syndrome' is a severe form of polymyositis in which the myositic inflammation is acute and aggressive onset, with common myalgias and cardiac involvement. Normally there is a poor reaction to therapy with a 5-year survival rate of about 25%.
SRP54 is a full-length cDNA coding for the human SRP54 protein having a molecular mass of 62kDa (pH 8.9). SRP54 protein is fused to a hexa-histidine purification tag.
| UniProt Protein Function: | SRP54: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Belongs to the GTP-binding SRP family. SRP54 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 14q13.2 Cellular Component: cytoplasm; signal recognition particle, endoplasmic reticulum targeting; nucleolus; nuclear speck; nucleus; cytosol Molecular Function:GTPase activity; ribonucleoprotein binding; GDP binding; GTP binding; drug binding; endoplasmic reticulum signal peptide binding; 7S RNA binding Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane, translocation; SRP-dependent cotranslational protein targeting to membrane; protein targeting to ER; cellular protein metabolic process; translation; SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; gene expression |
| UniProt Code: | P61011 |
| NCBI GenInfo Identifier: | 46577650 |
| NCBI Gene ID: | 6729 |
| NCBI Accession: | P61011.1 |
| UniProt Secondary Accession: | P61011,P14576, |
| UniProt Related Accession: | P61011 |
| Molecular Weight: | 504 |
| NCBI Full Name: | Signal recognition particle 54 kDa protein |
| NCBI Synonym Full Names: | signal recognition particle 54kDa |
| NCBI Official Symbol: | SRP54�� |
| NCBI Protein Information: | signal recognition particle 54 kDa protein; signal recognition particle 54kD |
| UniProt Protein Name: | Signal recognition particle 54 kDa protein |
| Protein Family: | Signal recognition particle |
| UniProt Gene Name: | SRP54�� |
| UniProt Entry Name: | SRP54_HUMAN |
