Description
| Product Name: | Human SPRY4 Recombinant Protein |
| Product Code: | RPPB5922 |
| Size: | 25µg |
| Species: | Human |
| Target: | SPRY4 |
| Synonyms: | Protein sprouty homolog 4, SPRY4, Sprouty Homolog 4, Spry-4, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila). |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SPRY4 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YHCTNEDDEG SCADHPCSCS RSNCCARWSF MGALSVVLPC LLCYLPATGC VKLAQRGYDR LRRPGCRCKH TNSVICKAAS GDAKTSRPDK PF |
Sprouty Homolog 4 (SPRY4) is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. SPRY4 is positioned upstream of RAS activation. SPRY4 protein functions include suppressing�the EGFR-transduced MAPK signaling pathway as well as impairing the formation of GTP-Ras.
SPRY4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-299) and having a molecular mass of 34.9 kDa.SPRY4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SPRY4: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Belongs to the sprouty family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor; Adaptor/scaffold Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; focal adhesion; Golgi apparatus Molecular Function:protein binding Disease: Hypogonadotropic Hypogonadism 17 With Or Without Anosmia |
| NCBI Summary: | This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014] |
| UniProt Code: | Q9C004 |
| NCBI GenInfo Identifier: | 14916719 |
| NCBI Gene ID: | 81848 |
| NCBI Accession: | Q9C004.2 |
| UniProt Secondary Accession: | Q9C004,Q6QIX2, Q9C003, A4FVB2, A4FVB3, |
| UniProt Related Accession: | Q9C004 |
| Molecular Weight: | 11,482 Da |
| NCBI Full Name: | Protein sprouty homolog 4 |
| NCBI Synonym Full Names: | sprouty RTK signaling antagonist 4 |
| NCBI Official Symbol: | SPRY4�� |
| NCBI Official Synonym Symbols: | HH17�� |
| NCBI Protein Information: | protein sprouty homolog 4 |
| UniProt Protein Name: | Protein sprouty homolog 4 |
| Protein Family: | Protein sprouty |
| UniProt Gene Name: | SPRY4�� |
| UniProt Entry Name: | SPY4_HUMAN |
