Description
| Product Name: | Human SOCS3 Recombinant Protein |
| Product Code: | RPPB4770 |
| Size: | 10µg |
| Species: | Human |
| Target: | SOCS3 |
| Synonyms: | Suppressor Of Cytokine Signaling 3, CIS3, SSI3, Cytokine-InducibleSH2 Protein 3, STAT-Induced STAT Inhibitor 3, ATOD4, Cish3, MGC71791, CIS-3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | SOCS3 protein solution (0.5mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMVT HSKFPAAGMS RPLDTSLRLK TFSSKSEYQL VVNAVRKLQESGFYWSAVTG GEANLLLSAE PAGTFLIRDS SDQRHFFTLS VKTQSGTKNL RIQCEGGSFS LQSDPRSTQPVPRFDCVLKL VHHYMPPPGA PSFPSPPTEP SSEVPEQPSA QPLPGSPPRR AYYIYSGGEK IPLVLSRPLSSNVATLQHLC RKTVNGHLDS YEKVTQLPGP IREFLDQYDA PL |
Suppressor of Cytokine Signaling 3, also known as SOCS3 belongs to the SOCS family; in addition SOCS3 is a member of the STAT-induced STAT inhibitor (SSI). The SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of SOCS3 is induced by different cytokines, including IL6 and IL10. SOCS3 can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of SOCS3 have shown the roles of SOCS3 in the negative regulation of fetal liver hematopoiesis, and placental development.
SOCS3 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 262 amino acids (1-225 a.a) andhaving a molecular mass of 29kDa.SOCS3 is fused to a 37 amino acid His-tag atN-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: regulation of growth; negative regulation of inflammatory response; cytokine and chemokine mediated signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; negative regulation of insulin receptor signaling pathway; JAK-STAT cascade; positive regulation of cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of apoptosis Disease: Dermatitis, Atopic, 4 |
| NCBI Summary: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14543 |
| NCBI GenInfo Identifier: | 20178094 |
| NCBI Gene ID: | 9021 |
| NCBI Accession: | O14543.1 |
| UniProt Secondary Accession: | O14543,O14509, |
| UniProt Related Accession: | O14543 |
| Molecular Weight: | |
| NCBI Full Name: | Suppressor of cytokine signaling 3 |
| NCBI Synonym Full Names: | suppressor of cytokine signaling 3 |
| NCBI Official Symbol: | SOCS3�� |
| NCBI Official Synonym Symbols: | CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3�� |
| NCBI Protein Information: | suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3 |
| UniProt Protein Name: | Suppressor of cytokine signaling 3 |
| UniProt Synonym Protein Names: | Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3 |
| Protein Family: | Suppressor of cytokine signaling |
| UniProt Gene Name: | SOCS3�� |
| UniProt Entry Name: | SOCS3_HUMAN |
