Description
| Product Name: | Human SNPH Recombinant Protein |
| Product Code: | RPPB4745 |
| Size: | 50µg |
| Species: | Human |
| Target: | SNPH |
| Synonyms: | KIAA0374, MGC46096, bA314N13.5, SNPH, Syntaphilin. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The Syntaphilin protein solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAMSLPGSRR TSAGSRRRTS PPVSVRDAYG TSSLSSSSNS GSYKGSDSSP TPRRSMKYTL CSDNHGIKPPTPEQYLTPLQ QKEVCIRHLK ARLKDTQDRL QDRDTEIDDL KTQLSRMQED WIEEECHRVE AQLALKEARK EIKQLKQVID TVKNNLIDKDKGLQKYFVDI NIQNKKLETL LHSMEVAQNG MAKEDGTGES AGGSPARSLT RSSTYTKLSD PAVCGDRQPG DPSSGSAEDG ADSGFAAADDTLSRTDALEA SSLLSSGVDC GTEETSLHSS FGLGPRFPAS NTYEKLLCGM EAGVQASCMQ ERAIQTDFVQ YQPDLDTILE KVTQAQVCGTDPESGDRCPE LDAHPSGPRD PNSAVVVTVG DELEAPEPIT RGPTPQRPGA NPNPGQSVSV VCPMEEEEEA AVAEKEPKSY WSRH |
Syntaxin-1, synaptobrevin, and SNAP25 cooperate to form the SNARE complex, which is needed for synaptic vesicle docking and fusion SNPH is a neuron-specific protein originally characterized as a binding partner of syntaxin-1. SNPH participates with SNAP25 for the binding to Syntaxin-1 and prevents the construction of the SNARE core complex, thus manageling free syntaxin-1 availability for the assembly of the SNARE complex and potentially regulating synaptic vesicle exocytosis. Expression Syntaphilin appears to be brain-specific. SNPH is an inhibitor of both SNARE-based fusion and dynamin-mediated endocytosis.
SNPH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 444 amino acids (1-424) and having a molecular mass of 48.2 kDa.The SNPH is fused to 20 amino acid His-Tag at N-terminus and purified by standard chromatography techniques.
| UniProt Protein Function: | syntaphilin: Inhibits SNARE complex formation by absorbing free syntaxin-1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Vesicle Chromosomal Location of Human Ortholog: 20p13 Cellular Component: presynaptic membrane; neuron projection; cell soma; cytoplasmic microtubule; mitochondrial membrane; cytoplasm; integral to membrane; cell junction Molecular Function:syntaxin-1 binding Biological Process: neuron differentiation; neurotransmitter secretion; brain development; synaptic vesicle docking during exocytosis |
| NCBI Summary: | Syntaxin-1, synaptobrevin/VAMP, and SNAP25 interact to form the SNARE complex, which is required for synaptic vesicle docking and fusion. The protein encoded by this gene is membrane-associated and inhibits SNARE complex formation by binding free syntaxin-1. Expression of this gene appears to be brain-specific. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15079 |
| NCBI GenInfo Identifier: | 21362912 |
| NCBI Gene ID: | 9751 |
| NCBI Accession: | O15079.1 |
| UniProt Secondary Accession: | O15079,Q8IYI3, |
| UniProt Related Accession: | O15079 |
| Molecular Weight: | 57,990 Da |
| NCBI Full Name: | Syntaphilin |
| NCBI Synonym Full Names: | syntaphilin |
| NCBI Official Symbol: | SNPH�� |
| NCBI Official Synonym Symbols: | bA314N13.5�� |
| NCBI Protein Information: | syntaphilin |
| UniProt Protein Name: | Syntaphilin |
| Protein Family: | Syntaphilin |
| UniProt Gene Name: | SNPH�� |
| UniProt Entry Name: | SNPH_HUMAN |
