Description
| Product Name: | Human SMAD2 Recombinant Protein |
| Product Code: | RPPB4714 |
| Size: | 10µg |
| Species: | Human |
| Target: | SMAD2 |
| Synonyms: | JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, MGC22139, MGC34440, SMAD2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad-related protein 2, SMAD 2, SMAD family member 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SMAD2 protein solution contains 20mM Tris-HCl pH-8, and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPSTCSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLVPRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYYELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGCNLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS |
SMAD2 is part of the SMAD family of proteins that mediate signal transduction by the TGF-beta/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated SMAD2 is then able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. TGF-beta 1 acts on adjacent stromal cells to turn on SMAD2 signalling that could lead to stromal decidualization. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells.
SMAD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 487 amino acids (1-467) and having a molecular mass of 54.4 kDa. SMAD2 is fused to 20 amino acid His-Tag at N-Terminus and purified by standard chromatography techniques.
| UniProt Protein Function: | SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus; cytosol Molecular Function:transcription activator binding; metal ion binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transforming growth factor beta receptor binding; transcription factor activity Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; palate development; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; pancreas development; response to glucose stimulus; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; pericardium development; cell fate commitment; regulation of binding; transcription, DNA-dependent; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; endoderm formation; insulin secretion; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development |
| NCBI Summary: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q15796 |
| NCBI GenInfo Identifier: | 13633914 |
| NCBI Gene ID: | 4087 |
| NCBI Accession: | Q15796.1 |
| UniProt Related Accession: | Q15796 |
| Molecular Weight: | 52kDa |
| NCBI Full Name: | Mothers against decapentaplegic homolog 2 |
| NCBI Synonym Full Names: | SMAD family member 2 |
| NCBI Official Symbol: | SMAD2�� |
| NCBI Official Synonym Symbols: | JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2�� |
| NCBI Protein Information: | mothers against decapentaplegic homolog 2; MAD homolog 2; mother against DPP homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2 |
| UniProt Protein Name: | Mothers against decapentaplegic homolog 2 |
| UniProt Synonym Protein Names: | JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2 |
| Protein Family: | Mothers against decapentaplegic |
| UniProt Gene Name: | SMAD2�� |
| UniProt Entry Name: | SMAD2_HUMAN |
