Description
| Product Name: | Human SH3GLB1 Recombinant Protein |
| Product Code: | RPPB4676 |
| Size: | 20µg |
| Species: | Human |
| Target: | SH3GLB1 |
| Synonyms: | Bif-1, CGI-61, dJ612B15.2, PPP1R70, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, KIAA0491 . |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SH3GLB1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE CTKIWTEKIM KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY MIDAGTEFGP GTAYGNALIK CGETQKRIGT ADRELIQTSA LNFLTPLRNF IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK KAKAAETRNS SEQELRITQS EFDRQAEITR LLLEGISSTH AHHLRCLNDF VEAQMTYYAQ CYQYMLDLQK QLGSFPSNYL SNNNQTSVTP VPSVLPNAIG SSAMASTSGL VITSPSNLSD LKECSGSRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLNLEHHH HHH |
Endophilin-B1 (SH3GLB1) is a part of the endophilin family and highly expresses in heart, skeletal muscle, kidney and placenta. Endophilin B1 belongs to the B subgroup of the endophilin family which is needed for preservation of mitochondrial morphology and for the regulation of the outer mitochondrial membrane dynamics. SH3GLB1 is required for normal outer mitochondrial membrane dynamics. Furthermore, SH3GLB1 is required for coatomer-mediated retrograde transport in certain cells. SH3GLB1 interacts with SH3GLB2 and Bcl-2-associated X protein and involved in regulating apoptotic signaling pathways.
SH3GLB1 Human Recombinant produced in E. coli is a single polypeptide chain containing 373 amino acids (1-365) and having a molecular mass of 41.9 kDa. SH3GLB1 is fused to 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis; EC 2.3.1.-; Autophagy; Transferase Chromosomal Location of Human Ortholog: 1p22 Cellular Component: cytoplasm; midbody Molecular Function:identical protein binding; protein binding; protein homodimerization activity Biological Process: autophagic cell death; cellular response to amino acid starvation; cellular response to glucose starvation; positive regulation of autophagy; positive regulation of protein oligomerization; protein oligomerization; receptor catabolic process; regulation of cytokinesis; regulation of macroautophagy; regulation of protein stability |
| NCBI Summary: | This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q9Y371 |
| NCBI GenInfo Identifier: | 41018158 |
| NCBI Gene ID: | 51100 |
| NCBI Accession: | Q9Y371.1 |
| UniProt Secondary Accession: | Q9Y371,Q5H8U5, Q9H3Z0, Q9NR47, Q9NYA9, B4E182, |
| UniProt Related Accession: | Q9Y371 |
| Molecular Weight: | 29,318 Da |
| NCBI Full Name: | Endophilin-B1 |
| NCBI Synonym Full Names: | SH3 domain containing GRB2 like endophilin B1 |
| NCBI Official Symbol: | SH3GLB1�� |
| NCBI Official Synonym Symbols: | Bif-1; CGI-61; PPP1R70; dJ612B15.2�� |
| NCBI Protein Information: | endophilin-B1 |
| UniProt Protein Name: | Endophilin-B1 |
| UniProt Synonym Protein Names: | Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1 |
| Protein Family: | Endophilin |
| UniProt Gene Name: | SH3GLB1�� |
| UniProt Entry Name: | SHLB1_HUMAN |
