Description
| Product Name: | Human SFRP4 Recombinant Protein |
| Product Code: | RPPB4655 |
| Size: | 10µg |
| Species: | Human |
| Target: | SFRP4 |
| Synonyms: | Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE, FRP-4. |
| Source: | HEK293 Cells |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4�m) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility: | It is recommended to add 200�l deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. SFRP4 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | PGDYKDDDDK PAGVRGAPCE AVRIPMCRHM PWNITRMPNH LHHSTQENAI LAIEQYEELV DVNCSAVLRF FLCAMYAPIC TLEFLHDPIK PCKSVCQRAR DDCEPLMKMY NHSWPESLAC DELPVYDRGV CISPEAIVTD LPEDVKWIDI TPDMMVQERP LDVDCKRLSP DRCKCKKVKP TLATYLSKNY SYVIHAKIKA VQRSGCNEVT TVVDVKEIFK SSSPIPRTQV PLITNSSCQC PHILPHQDVL IMCYEWRSRM MLLENCLVEK WRDQLSKRSI QWEERLQEQR RTVQDKKKTA GRTSRSNPPK PKGKPPAPKP ASPKKNIKTR SAQKRTNPKR V |
Secreted frizzled-related protein 4 (SFRP4) belongs to the SFRP family which contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs serve as soluble modulators of Wnt signaling. SFRP4 may serve as a regulator of adult uterine morphology and function. SFRP4 increases apoptosis during ovulation possibly via modulation of FZ1/FZ4/WNT4 signaling. SFRP4 also has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. SFRP4 is expressed in proliferative endometrium and several types of ovarian, endometrial and Brest tumors. SFRP4 is expressed in mesenchymal cells and in cardiomyocytes. SFRP4 expression in ventricular myocardium correlates with apoptosis related gene expression. SFRP4 is up-regulated in failing myocardium.
SFRP4 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 19-346) containing a total of 341 amino acids, having a molecular mass of 39kDa (calculated), though it migrates at approximately 55kDa on SDS PAGE, the SFRP4 is fused to a 5 a.a N-terminal linker and an 8 a.a Flag tag at N-Terminus.The Human SFRP4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 7p14.1 Cellular Component: cell surface; cytoplasm; extracellular space; integral to membrane; nucleus Molecular Function:G-protein coupled receptor activity; protein binding; Wnt receptor activity; Wnt-protein binding Biological Process: negative regulation of cell proliferation; negative regulation of transcription factor activity; phosphate ion homeostasis; positive regulation of apoptosis; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization |
| NCBI Summary: | Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6FHJ7 |
| NCBI GenInfo Identifier: | 61216780 |
| NCBI Gene ID: | 6424 |
| NCBI Accession: | Q6FHJ7.2 |
| UniProt Secondary Accession: | Q6FHJ7,O14877, Q05BG7, Q1ZYW2, Q4G124, Q6FHM0, Q6PD64 B4DYC1, |
| UniProt Related Accession: | Q6FHJ7 |
| Molecular Weight: | 39,827 Da |
| NCBI Full Name: | Secreted frizzled-related protein 4 |
| NCBI Synonym Full Names: | secreted frizzled related protein 4 |
| NCBI Official Symbol: | SFRP4�� |
| NCBI Official Synonym Symbols: | PYL; FRP-4; FRPHE; sFRP-4�� |
| NCBI Protein Information: | secreted frizzled-related protein 4 |
| UniProt Protein Name: | Secreted frizzled-related protein 4 |
| UniProt Synonym Protein Names: | Frizzled protein, human endometrium; FrpHE |
| Protein Family: | Secreted frizzled-related protein |
| UniProt Gene Name: | SFRP4�� |
| UniProt Entry Name: | SFRP4_HUMAN |
