Description
| Product Name: | Human SERPINB5 Recombinant Protein |
| Product Code: | RPPB4639 |
| Size: | 25µg |
| Species: | Human |
| Target: | SERPINB5 |
| Synonyms: | PI5, maspin, SERPINB5, serpin peptidase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SERPINB5 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.� |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by�SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKILVVNAAYFVGK WMKKFPESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIMELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQLNSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP |
SERPINB5 (Maspin) is a tumor suppressor protein of the serine proteinase inhibitor family. Maspin plays a vital role in embryonic development through critical functions in cell adhesion. In addition, Maspin is present in normal breast and prostatic epithelial cells although down regulated in the particular carcinomas. SERPINB5 impedes the growth, invasion, and metastatic properties of mammary tumors as well as the invasive ability of pancreatic ductal adenocarcinoma cells. SERPINB5 being a breast tumor suppressor gene is a significant marker of the disease progression in breast neoplasms. Furthermore, high expression of maspin is linked to squamous cell carcinoma in non-small-cell lung cancer. Moreover, maspin expression has been directly linked with the biological aggressiveness of ovarian carcinoma. Maspin exhibits no serine protease inhibitory activity since it does not undergo the stressed to relaxed conformational transition typical of active serpins.
SERPINB5 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 395 amino acids (1-375 a.a.) and having a molecular mass of 44.2 kDa.The SERPINB5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 18q21.33 Cellular Component: extracellular space; cytoplasm Molecular Function:serine-type endopeptidase inhibitor activity; protein binding Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation |
| UniProt Code: | P36952 |
| NCBI GenInfo Identifier: | 229462757 |
| NCBI Gene ID: | 5268 |
| NCBI Accession: | P36952.2 |
| UniProt Secondary Accession: | P36952,Q6N0B4, Q8WW89, B2R6Y4, |
| UniProt Related Accession: | P36952 |
| Molecular Weight: | 25,763 Da |
| NCBI Full Name: | Serpin B5 |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade B (ovalbumin), member 5 |
| NCBI Official Symbol: | SERPINB5�� |
| NCBI Official Synonym Symbols: | PI5; maspin�� |
| NCBI Protein Information: | serpin B5; PI-5; peptidase inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 |
| UniProt Protein Name: | Serpin B5 |
| UniProt Synonym Protein Names: | Maspin; Peptidase inhibitor 5; PI-5 |
| Protein Family: | Serpin |
| UniProt Gene Name: | SERPINB5�� |
| UniProt Entry Name: | SPB5_HUMAN |
