Description
| Product Name: | Human SERPINB2 Recombinant Protein |
| Product Code: | RPPB4634 |
| Size: | 10µg |
| Species: | Human |
| Target: | SERPINB2 |
| Synonyms: | Serpin Peptidase Inhibitor Clade B (Ovalbumin) Member 2, Serine (Or Cysteine) Proteinase Inhibitor Clade B (Ovalbumin) Member 2, Placental Plasminogen Activator Inhibitor, Plasminogen Activator Inhibitor Type II (Arginine-Serpin), PAI2, PLANH2, Monocyte Arg-Serpin, Serpin B2, HsT1201, PAI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM Cysteine, with 5% Trehalose. |
| Solubility: | It is recommended to reconstitute the lyophilized SERPINB2 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized SERPINB2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SERPINB2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP� |
| Biological Activity: | The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37�C. Half maximal inhibition against 1.0 �g/ml of single chain tPA was at a concentration of 1.0�g/ml.� |
SERPINB2 is an inhibitory serpin produced primarily in keratinocytes, stimulated monocytes, and placental trophoblasts. SERPINB2 is found primarily as a 47 kDa non-glycosylated intracellular protein that is induced to be secreted as 60 kDa glycoprotein. The glycosylated and unglycosylated SERPINB2 are similarly effective as inhibitors of uPA, the only proven physiological target of SERPINB2.
SERPINB2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 415 amino acids and having a molecular mass of 46.6kDa.The SERPINB2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SERPINB2: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Belongs to the serpin family. Ov-serpin subfamily. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 18q21.3 Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: fibrinolysis; blood coagulation; negative regulation of apoptosis |
| UniProt Code: | P05120 |
| NCBI GenInfo Identifier: | 1352712 |
| NCBI Gene ID: | 5055 |
| NCBI Accession: | P05120.2 |
| UniProt Secondary Accession: | P05120,Q96E96, |
| UniProt Related Accession: | P05120 |
| Molecular Weight: | 46,596 Da |
| NCBI Full Name: | Plasminogen activator inhibitor 2 |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade B (ovalbumin), member 2 |
| NCBI Official Symbol: | SERPINB2�� |
| NCBI Official Synonym Symbols: | PAI; PAI2; PAI-2; PLANH2; HsT1201�� |
| NCBI Protein Information: | plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 |
| UniProt Protein Name: | Plasminogen activator inhibitor 2 |
| UniProt Synonym Protein Names: | Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor |
| UniProt Gene Name: | SERPINB2�� |
| UniProt Entry Name: | PAI2_HUMAN |
