Description
| Product Name: | Human S100P Recombinant Protein |
| Product Code: | RPPB4577 |
| Size: | 10µg |
| Species: | Human |
| Target: | S100P |
| Synonyms: | Protein S100-P, S100 calcium-binding protein P, S100P, S100E, MIG9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Filtered (0.4�m) and lyophilized from 0.5 mg/ml in 0.05M Phosphate buffer pH7.2, 0.1M NaCl. |
| Solubility: | Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MTELEAAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK |
S100P was originally described as a placental protein of 95 amino acid residues shares about 50% sequence identity with the brain S100 proteins alpha and beta.S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. S100P in addition to binding Ca2+, also binds Zn2+ and Mg2+. S100P gene is located on chromosome 4p16.S100P is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R, DU145, and PC3 and may play a role in the etiology of prostate cancer.In ductal hyperplasias, in situ and invasive ductal carcinoma, but not in the normal tissues, S100P overexpression is an early event that might play an important role in the immortalization of human breast epithelial cells in vitro and tumor progression in vivo.In NIH3T3 cells, the expression of S100P led to the presence of S100P in the culture medium, increased cellular proliferation, and enhanced survival following detachment from the culture substrate or after exposure to the chemotherapeutic agent 5-flurouracil. The proliferation and survival effects of S100P expression were duplicated in a time- and concentration-dependent manner by extracellular addition of purified S100P to wild-type NIH3T3 cells and correlated with the activation of Erks and NFB.To determine the mechanisms involved in these effects, we tested the hypothesis that S100P activated RAGE (Receptor for Activated Glycation End-Products). It was found that S100P coimmunoprecipitated with RAGE. Furthermore, the effects of S100P on cell signaling, proliferation and survival were blocked by agents that interfere with RAGE including administration of an amphoterin derived peptide known to antagonize RAGE activation, anti-RAGE antibodies and by expression of a dominant negative RAGE. These data suggest that S100P can act in an autocrine manner via RAGE to stimulate cell proliferation and survival.
The Recombinant Human S100P has a molecular mass of 10.4 kDa containing 95 amino acid residues of the human S100P.
| UniProt Protein Function: | May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE). |
| NCBI Summary: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25815 |
| NCBI GenInfo Identifier: | 134142 |
| NCBI Gene ID: | 6286 |
| NCBI Accession: | P25815.2 |
| UniProt Secondary Accession: | P25815,Q5J7W2, |
| UniProt Related Accession: | P25815 |
| Molecular Weight: | 10,400 Da |
| NCBI Full Name: | Protein S100-P |
| NCBI Synonym Full Names: | S100 calcium binding protein P |
| NCBI Official Symbol: | S100P�� |
| NCBI Official Synonym Symbols: | MIG9�� |
| NCBI Protein Information: | protein S100-P |
| UniProt Protein Name: | Protein S100-P |
| UniProt Synonym Protein Names: | Migration-inducing gene 9 protein; MIG9; Protein S100-E; S100 calcium-binding protein P |
| Protein Family: | Protein |
| UniProt Gene Name: | S100P�� |
| UniProt Entry Name: | S100P_HUMAN |
