Description
| Product Name: | Human RPRD1A Recombinant Protein |
| Product Code: | RPPB4504 |
| Size: | 20µg |
| Species: | Human |
| Target: | RPRD1A |
| Synonyms: | Regulation of Nuclear Pre-MRNA Domain Containing 1A, RPRD1A, P15RS, Cyclin-Dependent Kinase 2B-Inhibitor-Related Protein, Cyclin-Dependent Kinase Inhibitor 2B-Related Protein (P15INK4B-Related Protein), P15INK4B-Related Protein, HsT3101, Regulation of Nuclear Pre-MRNA Domain-Containing Protein 1A, Cyclin-Dependent Kinase Inhibitor 2B-Related Protein, FLJ10656. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RPRD1A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED |
Regulation Of Nuclear Pre-MRNA Domain Containing 1A (RPRD1A) is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a part in cell cycle regulation. RPRD1A interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and partakes in dephosphorylation of the CTD. RPRD1A May function as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.
RPRD1A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-312) and having a molecular mass of 38.4 kDa.RPRD1A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle. |
| NCBI Summary: | This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q96P16 |
| NCBI GenInfo Identifier: | 21361709 |
| NCBI Gene ID: | 55197 |
| NCBI Accession: | NP_060640 |
| UniProt Secondary Accession: | Q96P16,Q7Z5G8, Q96FY9, Q9NVL4, A8KA42, B2RBA3, |
| UniProt Related Accession: | Q96P16 |
| Molecular Weight: | 38.4kDa (337aa) confirmed by MALDI-TOF |
| NCBI Full Name: | regulation of nuclear pre-mRNA domain-containing protein 1A isoform 1 |
| NCBI Synonym Full Names: | regulation of nuclear pre-mRNA domain containing 1A |
| NCBI Official Symbol: | RPRD1A�� |
| NCBI Official Synonym Symbols: | P15RS; HsT3101�� |
| NCBI Protein Information: | regulation of nuclear pre-mRNA domain-containing protein 1A |
| UniProt Protein Name: | Regulation of nuclear pre-mRNA domain-containing protein 1A |
| UniProt Synonym Protein Names: | Cyclin-dependent kinase inhibitor 2B-related protein; p15INK4B-related protein |
| UniProt Gene Name: | RPRD1A�� |
