Description
| Product Name: | Human RPL8 Recombinant Protein |
| Product Code: | RPPB4490 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPL8 |
| Synonyms: | 60S ribosomal protein L8, RPL8, L8. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | RPL8 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 50% glycerol, 300mM NaCl and 2mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN |
60S ribosomal protein L8 (RPL8) is a member of the ribosomal protein L2P family. Ribosomes consist of a small 40S subunit and a large 60S subunit. RPL8 is found in the cytoplasm and exists as a component of the 60S subunit where it is assumed to have role in aminoacyl-tRNA binding, specifically at the ribosomal subunit interface. In rats, the RPL8 protein connects with the 5.8S rRNA, and possibly participates in the binding of aminoacyl-tRNA, and is a component of the elongation factor 2-binding site at the ribosomal subunit interface.
RPL8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-257 a.a) and having a molecular mass of 30.2kDa.RPL8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. |
| UniProt Protein Details: | Protein type:RNA-binding; Translation; Ribosomal Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus Molecular Function:RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62917 |
| NCBI GenInfo Identifier: | 51702823 |
| NCBI Gene ID: | 6132 |
| NCBI Accession: | P62917.2 |
| UniProt Secondary Accession: | P62917,P25120, Q567Q7, Q969V7, Q9BWQ9, A8K094, D3DWN2 |
| UniProt Related Accession: | P62917 |
| Molecular Weight: | 28,025 Da |
| NCBI Full Name: | 60S ribosomal protein L8 |
| NCBI Synonym Full Names: | ribosomal protein L8 |
| NCBI Official Symbol: | RPL8�� |
| NCBI Official Synonym Symbols: | L8�� |
| NCBI Protein Information: | 60S ribosomal protein L8 |
| UniProt Protein Name: | 60S ribosomal protein L8 |
| Protein Family: | 60S ribosomal protein |
| UniProt Gene Name: | RPL8�� |
| UniProt Entry Name: | RL8_HUMAN |
