Description
| Product Name: | Human RPL5 Recombinant Protein |
| Product Code: | RPPB4488 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPL5 |
| Synonyms: | 60S ribosomal protein L5, RPL5, MSTP030, Ribosomal protein L5, DBA6, L5, MSTP030. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | RPL5 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGFVKVV KNKAYFKRYQ VKFRRRREGK TDYYARKRLV IQDKNKYNTP KYRMIVRVTN RDIICQIAYA RIEGDMIVCA AYAHELPKYG VKVGLTNYAA AYCTGLLLAR RLLNRFGMDK IYEGQVEVTG DEYNVESIDG QPGAFTCYLD AGLARTTTGN KVFGALKGAV DGGLSIPHST KRFPGYDSES KEFNAEVHRK HIMGQNVADY MRYLMEEDED AYKKQFSQYI KNSVTPDMME EMYKKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES |
Ribosomal Protein L5, also known as RPL5 is a part of the ribosomal protein L18P family. RPL5 which binds 5S RNA is necessary for rRNA maturation and structure of the 60S ribosomal subunits.
RPL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-297 a.a.) and having a molecular mass of 36.8kDa.RPL5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RPL5: Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA. Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6). DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L18P family. |
| UniProt Protein Details: | Protein type:Ribosomal; Translation; Nucleolus Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; ribonucleoprotein complex Molecular Function:5S rRNA binding; protein binding; RNA binding; structural constituent of ribosome Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; ribosomal large subunit assembly and maintenance; ribosomal large subunit biogenesis and assembly; rRNA processing; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription Disease: Diamond-blackfan Anemia 6 |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46777 |
| NCBI GenInfo Identifier: | 81175191 |
| NCBI Gene ID: | 6125 |
| NCBI Accession: | P46777.3 |
| UniProt Secondary Accession: | P46777,Q32LZ3, Q53HH6, Q9H3F4, |
| UniProt Related Accession: | P46777 |
| Molecular Weight: | 34,363 Da |
| NCBI Full Name: | 60S ribosomal protein L5 |
| NCBI Synonym Full Names: | ribosomal protein L5 |
| NCBI Official Symbol: | RPL5�� |
| NCBI Official Synonym Symbols: | L5; DBA6; MSTP030; PPP1R135�� |
| NCBI Protein Information: | 60S ribosomal protein L5 |
| UniProt Protein Name: | 60S ribosomal protein L5 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL5�� |
| UniProt Entry Name: | RL5_HUMAN |
