Description
| Product Name: | Human RNF7 Recombinant Protein |
| Product Code: | RPPB5915 |
| Size: | 20µg |
| Species: | Human |
| Target: | RNF7 |
| Synonyms: | RING-box protein 2 isoform 1, Ring finger protein 7, CKBBP1, ROC2, SAG, RING-box protein 2, Rbx2, CKII beta-binding protein 1, Regulator of cullins 2, Sensitive to apoptosis gene protein, RBX2, RNF7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RNF7 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK |
Ring Finger Protein 7, also known as� RNF7, is an extremely conserved ring finger protein. RNF7 is a vital subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases that are a part of the protein degradation machinery important for cell cycle progression and signal transduction. RNF7 is a substrate of casein kinase II (CSNK2A1/CKII) and also interacts with it. The phosphorylation of RNF7 by CSNK2A1 promotes the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B).
RNF7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 136 amino acids (1-113 a.a) and having a molecular mass of 15.1kDa.RNF7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RNF7: a highly conserved ring finger protein. An essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. The RING-type zinc finger recruits the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. This protein interacts with, and is a substrate of, casein kinase II (CKII). The phosphorylation of this protein by CKII has been shown to promote the degradation of IkappaB-alpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Two alternatively spliced isoforms have been reported. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ligase; Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 3q23 Cellular Component: Cul5-RING ubiquitin ligase complex; cytosol; nucleoplasm Molecular Function:copper ion binding; NEDD8 ligase activity; protein binding Biological Process: protein neddylation; response to redox state |
| NCBI Summary: | The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UBF6 |
| NCBI GenInfo Identifier: | 37538003 |
| NCBI Gene ID: | 9616 |
| NCBI Accession: | Q9UBF6.1 |
| UniProt Secondary Accession: | Q9UBF6,Q9BXN8, Q9Y5M7, A8K1H9, A8MTB5, C9JYL3, D3DNF7 D3DNF8, |
| UniProt Related Accession: | Q9UBF6 |
| Molecular Weight: | 10,876 Da |
| NCBI Full Name: | RING-box protein 2 |
| NCBI Synonym Full Names: | ring finger protein 7 |
| NCBI Official Symbol: | RNF7�� |
| NCBI Official Synonym Symbols: | SAG; ROC2; CKBBP1�� |
| NCBI Protein Information: | RING-box protein 2 |
| UniProt Protein Name: | RING-box protein 2 |
| UniProt Synonym Protein Names: | CKII beta-binding protein 1; CKBBP1; RING finger protein 7; Regulator of cullins 2; Sensitive to apoptosis gene protein |
| UniProt Gene Name: | RNF7�� |
