Description
| Product Name: | Human Recoverin Recombinant Protein |
| Product Code: | RPPB4432 |
| Size: | 50µg |
| Species: | Human |
| Target: | Recoverin |
| Synonyms: | RCV1, Cancer-associated retinopathy protein, Protein CAR, RCVRN, Recoverin. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The protein (1mg/ml) contains 20mM Tris-HCl pH 8.0, 1mM EDTA, 2mM MgCl2 and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKNEVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA |
Recoverin is a member of the recoverin family of neuronal calcium sensors. Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction 23kDa protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recoverin may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin plays a key role in the inhibition of rhodopsin kinase, a molecule that regulates the phosphorylation of rhodopsin. This in due course controls the ability of the eye to adapt to, and recover from, exposure to the presence of light. Recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome.
Recoverin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids & having a molecular mass of 23kDa.
| UniProt Protein Function: | RCV1: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse. Belongs to the recoverin family. |
| UniProt Protein Details: | Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 17p13.1 Molecular Function:calcium ion binding; calcium sensitive guanylate cyclase activator activity Biological Process: phototransduction, visible light; positive regulation of guanylate cyclase activity; regulation of calcium ion transport; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; signal transduction; visual perception |
| NCBI Summary: | This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35243 |
| NCBI GenInfo Identifier: | 464600 |
| NCBI Gene ID: | 5957 |
| NCBI Accession: | P35243.2 |
| UniProt Secondary Accession: | P35243,Q53XL0, |
| UniProt Related Accession: | P35243 |
| Molecular Weight: | 23,130 Da |
| NCBI Full Name: | Recoverin |
| NCBI Synonym Full Names: | recoverin |
| NCBI Official Symbol: | RCVRN�� |
| NCBI Official Synonym Symbols: | RCV1�� |
| NCBI Protein Information: | recoverin |
| UniProt Protein Name: | Recoverin |
| UniProt Synonym Protein Names: | Cancer-associated retinopathy protein; Protein CAR |
| Protein Family: | Recoverin |
| UniProt Gene Name: | RCVRN�� |
| UniProt Entry Name: | RECO_HUMAN |
