Description
| Product Name: | Human RAP1A Recombinant Protein |
| Product Code: | RPPB4401 |
| Size: | 10µg |
| Species: | Human |
| Target: | RAP1A |
| Synonyms: | KREV1, G-22K, C21KG, RAP1A, RAP1, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | 0.25mg/ml solution containing 20mM Tris HCL pH-8.0, 5mM DTT, 0.2M NaCl & 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C |
RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristicsin common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner.
RAP1A Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The RAP1A is fused to 20 amino acid His-Tag at N-terminus and purified by conventional chromatography.
| UniProt Protein Function: | RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3. |
| UniProt Protein Details: | Protein type:Tumor suppressor; G protein, monomeric; Motility/polarity/chemotaxis; G protein; G protein, monomeric, Ras Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol Molecular Function:GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity Biological Process: platelet activation; microvillus biogenesis; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction |
| NCBI Summary: | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | P62834 |
| NCBI GenInfo Identifier: | 51338607 |
| NCBI Gene ID: | 5906 |
| NCBI Accession: | P62834.1 |
| UniProt Secondary Accession: | P62834,P10113, |
| UniProt Related Accession: | P62834 |
| Molecular Weight: | 184 |
| NCBI Full Name: | Ras-related protein Rap-1A |
| NCBI Synonym Full Names: | RAP1A, member of RAS oncogene family |
| NCBI Official Symbol: | RAP1A�� |
| NCBI Official Synonym Symbols: | RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21�� |
| NCBI Protein Information: | ras-related protein Rap-1A; Ras-related protein Krev-1; GTP-binding protein smg p21A |
| UniProt Protein Name: | Ras-related protein Rap-1A |
| UniProt Synonym Protein Names: | C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1 |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAP1A�� |
| UniProt Entry Name: | RAP1A_HUMAN |
