Description
| Product Name: | Human RANBP3 Recombinant Protein |
| Product Code: | RPPB4399 |
| Size: | 20µg |
| Species: | Human |
| Target: | RANBP3 |
| Synonyms: | RAN binding protein 3, RanBP3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RANBP3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSISSSLEN STNSADASSN KFVFGQNMSE RVLSPPKLNE VSSDANRENA AAESGSESSS QEATPEKESL AESAAAYTKA TARKCLLEKV EVITGEEAES NVLQMQCKLF VFDKTSQSWV ERGRGLLRLN DMASTDDGTL QSRLVMRTQG SLRLILNTKL WAQMQIDKAS EKSIRITAMD TEDQGVKVFL ISASSKDTGQ LYAALHHRIL ALRS |
RAN Binding Protein 3, also known as RANBP3, is found in the nucleus and cytoplasm. RANBP3 which owns RanBD1 domain participates in nuclear export as part of a heteromeric complex. RANBP3 is also negatively regulates TGF-beta signaling by interacting with the R-SMAD proteins, SMAD2 and SMAD3.
RANBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (235-445 a.a) and having a molecular mass of 25.5kDa. RANBP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RANBP3: Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF- beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling. Widely expressed with high levels in testis and heart. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: positive regulation of protein export from nucleus |
| NCBI Summary: | This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H6Z4 |
| NCBI GenInfo Identifier: | 51316528 |
| NCBI Gene ID: | 8498 |
| NCBI Accession: | Q9H6Z4.1 |
| UniProt Secondary Accession: | Q9H6Z4,O60405, O75759, O75760, Q9BT47, Q9UG74, B2RAT8 |
| UniProt Related Accession: | Q9H6Z4 |
| Molecular Weight: | 53,411 Da |
| NCBI Full Name: | Ran-binding protein 3 |
| NCBI Synonym Full Names: | RAN binding protein 3 |
| NCBI Official Symbol: | RANBP3�� |
| NCBI Protein Information: | ran-binding protein 3 |
| UniProt Protein Name: | Ran-binding protein 3 |
| Protein Family: | Ran-binding protein |
| UniProt Gene Name: | RANBP3�� |
| UniProt Entry Name: | RANB3_HUMAN |
