Description
| Product Name: | Human RANBP1 Recombinant Protein |
| Product Code: | RPPB4398 |
| Size: | 25µg |
| Species: | Human |
| Target: | RANBP1 |
| Synonyms: | RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RANBP1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ |
RANBP1 is a member of a family of proteins which bind Ran GTPase. Ran GTPase is vital for cell cycle control, mRNA processing, nuclear transport, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not stimulate GTPase activity of RAN however RANBP1 does distinctly raise GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). Additionally, RANBP1 operates as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
RANBP1 Human Recombinant produced in�E. coli is a single polypeptide chain containing 225 amino acids (1-201) and having a molecular mass of 25.8kDa (molecular size on SDS-PAGE will appear higher).RANBP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RANBP1: a Ran-binding protein that functions in nuclear trafficking, mitosis, chromosomal segregation, and ciliogenesis. Controls both nuclear import and export. Contains a Ran binding domain and a C-terminal nuclear export signal. Following export from the nucleus, forms a complex with RanGTP and XPO1/cargo, leading to dissociation of cargo from XPO1. Stimulates RanGAP1 association with RanGTP, facilitating RanGTP hydrolysis to RanGDP. Stabilizes and regulates the formation of a RanGDP-importin/NLS receptor-RanBP1 complex during nuclear import. Controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and DLG7, to mitotic microtubles and regulates chromosome segregation. Knock down or overexpression experiments affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia.Belongs to the RANBP1 family. |
| UniProt Protein Details: | Protein type:G protein regulator, misc.; Microtubule-binding; Cell cycle regulation Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytoplasm; nuclear envelope; nucleus Molecular Function:GDP-dissociation inhibitor activity; Ran GTPase binding; GTPase activator activity Biological Process: intracellular transport; viral reproduction; regulation of catalytic activity; signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P43487 |
| NCBI GenInfo Identifier: | 520975477 |
| NCBI Gene ID: | 5902 |
| NCBI Accession: | NP_001265568.1 |
| UniProt Related Accession: | P43487 |
| Molecular Weight: | |
| NCBI Full Name: | ran-specific GTPase-activating protein isoform 1 |
| NCBI Synonym Full Names: | RAN binding protein 1 |
| NCBI Official Symbol: | RANBP1�� |
| NCBI Official Synonym Symbols: | HTF9A�� |
| NCBI Protein Information: | ran-specific GTPase-activating protein |
| UniProt Protein Name: | Ran-specific GTPase-activating protein |
| UniProt Synonym Protein Names: | Ran-binding protein 1; RanBP1 |
| UniProt Gene Name: | RANBP1�� |
| UniProt Entry Name: | RANG_HUMAN |
